Evidence map›Paper›PMID 39758988›Full record

ArticleiScience2024

HRP-integrated CRISPR-Cas12a biosensor for rapid point-of-care detection of Langya henipavirus.

Xuan Wu, Rong Xiang, Danting Yang, Xiaoyao He, Lu Zhu, Fangfang Sun, Hanqing Li, Ningning Pi, Yi Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. One health (Amsterdam, Netherlands) · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xuan WuKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Rong XiangPrecision Medicine Center, The Second Affiliated Hospital of Chongqing Medical University, 288 Tianwen Road, Chongqing 400010, China.
Danting YangSchool of Public Health, Zhejiang Key Laboratory of Pathophysiology, Health Science Center, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang 315211, China.
Xiaoyao HeKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Lu ZhuKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Fangfang SunKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Hanqing LiKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Ningning PiKey Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing, Ecological and Environment Monitoring Center of Chongqing, 252 Qishan Road, Chongqing 401132, China.
Yi LiKey Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), School of Laboratory Medicine, Chongqing Medical University, 1 Xueyuan Road, Chongqing 400016, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Global pandemic has emphasized the needs for advanced pathogen diagnosis in dealing with newly emerged infectious threats, including the Langya henipavirus (LayV). LayV, as an emerging zoonotic pathogen, has potential to cause pandemic, but lacks of rapid diagnostic tools, particularly at point-of-care level. Here, we leveraged the merits of CRISPR-Cas12a biosensing and established a highly sensitive LayV detection method. By integrating CRISPR-Cas12a with RPA, 10 copies/μL ultra-sensitive LayV RNA detection has been achieved at room temperature within 30 min. More importantly, this study developed a special horseradish peroxidase (HRP)-single-stranded DNA (ssDNA) reporter enabling CRISPR-Cas12a to detect LayV RNA without pre-amplification, achieving a sensitivity of 1,200 copies/μL detectable by the naked eye. These explorations can serve as accelerator for CRISPR-Cas biosensing toward rapid response for newly emerged biological threats, and also provide a method to realize simple, precise, and amplicon-free point-of-care pathogen screening for resource limited or underdevelopment regions.

Indexed as

Biological sciencesImmunological methodsMicrobiologyNatural sciences

Identifiers

PMID39758988
PMCPMC11697611

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.