Evidence map›Paper›PMID 39758748›Full record

ArticleOpen forum infectious diseases2025

Dengue Virus Structural Proteins Are Expressed on the Surface of DENV-Infected Cells and Are a Target for Antibody-Dependent Cellular Phagocytosis.

Mitchell J Waldran, Elizabeth A Kurtz, Chad J Gebo, Timothy J Rooney, Frank A Middleton, Nathan H Roy, Jeffrey R Currier, Adam T Waickman

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PubMed Full text at PMC Publisher
In one paragraph

Article in Open forum infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mitchell J WaldranDepartment of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Elizabeth A KurtzDepartment of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Chad J GeboDepartment of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Timothy J RooneyDepartment of Neuroscience and Physiology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Frank A MiddletonDepartment of Neuroscience and Physiology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Nathan H RoyDepartment of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA.
Jeffrey R CurrierViral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.ORCID https://orcid.org/0000-0002-5696-0268
Adam T WaickmanDepartment of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA.ORCID https://orcid.org/0000-0002-4536-6714

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Dengue virus (DENV) is an arboviral pathogen found in >100 countries and a source of significant morbidity and mortality. While the mechanisms underpinning the pathophysiology of severe Dengue are incompletely understood, it has been hypothesized that antibodies directed against the DENV envelope (E) protein can facilitate antibody-dependent enhancement (ADE) of the infection, increasing the number of infected cells and the severity of disease in an exposed individual. Accordingly, there is interest in defining mechanisms for directly targeting DENV-infected cells for immunologic clearance, an approach that bypasses the risk of ADE. Methods: We have previously demonstrated that antibodies specific to DENV nonstructural protein 1 (NS1) can opsonize and facilitate the phagocytic clearance of DENV-infected cells. However, it is currently unclear if other DENV antigens are expressed on the surface of infected cells and if these antigens can be targeted by antibody-dependent clearance mechanisms. Results: In this study, we demonstrate that DENV structural proteins are expressed on the surface of DENV-infected cells and that these antigens can be opsonized by both DENV-immune sera and monoclonal antibodies. In addition, DENV E-specific antibodies can facilitate phagocytic uptake of material from DENV-infected cells, resulting in the target-cell membrane localizing to endosomes of the engulfing phagocyte. Notably, there was no selective enrichment of DENV genomic material in monocytes that had phagocytosed DENV-infected cell material compared with nonphagocytic monocytes. Discussion: In their totality, these data reinforce the concept that DENV E-reactive antibodies have a multifaceted role in DENV immunity and pathogenesis beyond neutralization and/or infection enhancement.

Indexed as

antibody-dependent cellular phagocytosisDengue virusDENVIgAIgG

Identifiers

PMID39758748
PMCPMC11697091

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.