ArticleACS synthetic biology2025
Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Impact of Reducing Agents on Protein Synthesis in a Reconstituted Cell-Free Protein Synthesis System.ACS synthetic biology · 2026Article
- Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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Authors and funding
4 authors.
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Abstract
Cell-free systems are powerful tools in synthetic biology with versatile and wide-ranging applications. However, a significant bottleneck for these systems, particularly the PURE cell-free system, is their limited reaction lifespan and yield. Dialysis offers a promising approach to prolong reaction lifetimes and increase yields, yet most custom dialysis systems require access to sophisticated equipment like 3D printers or microfabrication tools. In this study, we utilized an easy-to-assemble, medium-scale dialysis system for cell-free reactions using commercially available components. By employing dialysis with periodic exchange of the feeding solution, we achieved a protein yield of 1.16 mg/mL GFP in the PURE system and extended protein synthesis for at least 12.5 consecutive days, demonstrating the system's excellent stability.
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