Evidence map›Paper›PMID 39756884›Full record

ArticleThe Journal of veterinary medical science2025

Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing.

Nami Matsuda, Minori Ito, Yuka Nukada, Miyuki Toyoma, Kazuya Nagai, Tomoki Motegi, Tomoya Morita, Masahiro Yamasaki

Abstract read
In one paragraph

Article in The Journal of veterinary medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nami MatsudaLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.
Minori ItoLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.
Yuka NukadaLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.
Miyuki ToyomaLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.
Kazuya NagaiFaculty of Agriculture, Iwate University, Iwate, Japan.
Tomoki MotegiSection of Computational Biomedicine, Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Tomoya MoritaLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.
Masahiro YamasakiLaboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A comprehensive and quantitative method to compare gene expression may be useful for investigating the mechanisms responsible for diminazene aceturate (DA) resistance in Babesia gibsoni. Therefore, the gene expression of B. gibsoni cultured with DA was compared with those without DA using RNA sequencing (RNA-seq). Total RNA extracted from the parasites cultured with or without DA was examined using two next-generation sequencers, the 454 GS Junior and MiniSeq systems. We aimed to detect the genes differentially expressed between parasites cultured with and without DA by mapping the reads against de novo assembled contigs. The contigs, the amounts of which were more than five-fold higher in the parasite with DA than that without DA, were searched using BLAST

Indexed as

Antiprotozoal AgentsBabesiaDiminazeneAnimalsDrug ResistanceGene ExpressionSequence Analysis, RNAAntiprotozoal AgentsDiminazenediminazene aceturateBabesia gibsonidiminazene aceturate-resistantRNA-seq

Identifiers

PMID39756884
PMCPMC11830443

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.