ArticleScientific reports2025
Development of lateral flow immunochromatographic assay with Anti-Pythium insidiosum antibodies for point-of-care testing of vascular pythiosis.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Simplified Point-of-Care Testing for Human Pythiosis: Development of a Whole-Blood-Based Lateral Flow Immunoassay.Diagnostics (Basel, Switzerland) · 2026Article
- Enhancing knowledge of vascular pythiosis: Impact of a self-paced online course among Thai learners.PLoS neglected tropical diseases · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The pathogenic oomycete Pythium insidiosum causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis. However, these assays provide only indirect evidence of pythiosis and are not readily available in the commercial market. Here, we have developed an affordable point-of-care test (POCT) kit based on an immunochromatographic assay to detect P. insidiosum antigens directly. Our recent findings reveal that the lateral flow sandwich immunological testing cassette can accurately identify vascular pythiosis antigens using a small volume of patient's plasma, accomplishing diagnostic efficiency, with a limit of detection (LOD) of 8 ng/mL. This prototype cartridge represents a significant stride toward advancing enriched POCT for pythiosis serodiagnosis.
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