Evidence map›Paper›PMID 39754602›Full record

ArticleACS synthetic biology2025

ATP Regeneration from Pyruvate in the PURE System.

Surendra Yadav, Alexander J P Perkins, Sahan B W Liyanagedera, Anthony Bougas, Nadanai Laohakunakorn

Abstract read
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Frontiers in cell and developmental biology · 2025
    Review
  4. Synthetic cells for phage therapy: a perspective.Frontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Surendra YadavCentre for Engineering Biology, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.
Alexander J P PerkinsCentre for Engineering Biology, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.
Sahan B W LiyanagederaCentre for Engineering Biology, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.ORCID 0000-0002-9593-5487
Anthony BougasCentre for Engineering Biology, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.
Nadanai LaohakunakornCentre for Engineering Biology, Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.ORCID 0000-0002-6446-482X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The "Protein synthesis Using Recombinant Elements" ("PURE") system is a minimal biochemical system capable of carrying out cell-free protein synthesis using defined enzymatic components. This study extends PURE by integrating an ATP regeneration system based on pyruvate oxidase, acetate kinase, and catalase. The new pathway generates acetyl phosphate from pyruvate, phosphate, and oxygen, which is used to rephosphorylate ATP

Indexed as

Adenosine TriphosphatePyruvic AcidAcetate KinaseCatalaseCell-Free SystemEscherichia coliOrganophosphatesProtein BiosynthesisPyruvate OxidaseSynthetic BiologyAcetate Kinaseacetyl phosphateAdenosine TriphosphateCatalaseOrganophosphatesPyruvate OxidasePyruvic AcidATP regenerationcell-free protein synthesisPUREsynthetic biologysynthetic cellssynthetic metabolism

Identifiers

PMID39754602
PMCPMC11744923

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.