Evidence map›Paper›PMID 39754133›Full record

ArticleMicrobial cell factories2025

Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity.

Mervat G Hassan, Gharieb S El-Sayyad, Mohamed O Abdel-Monem, Mohamed N Malash, Mona A Kishk, Mohamed E El Awady, Mohamed I El-Khonezy

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Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mervat G HassanDepartment of Botany and Microbiology, Faculty of Science, Benha University, Benha, 33516, Egypt.ORCID http://orcid.org/0000-0002-5481-921X
Gharieb S El-SayyadMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Badr University in Cairo (BUC), Cairo, Egypt. Gharieb.El-Saied@buc.edu.eg.
Mohamed O Abdel-MonemDepartment of Botany and Microbiology, Faculty of Science, Benha University, Benha, 33516, Egypt.ORCID http://orcid.org/0000-0003-3403-253X
Mohamed N MalashDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University (ACU), Giza, Egypt.ORCID http://orcid.org/0000-0002-0200-9469
Mona A KishkDepartment of Botany and Microbiology, Faculty of Science, Benha University, Benha, 33516, Egypt.
Mohamed E El AwadyMicrobial Biotechnology Department, Biotechnology Research Institute, National Research Centre, El-Buhouth St. 33, Dokki, Cairo, Egypt.
Mohamed I El-KhonezyMolecular Biology Department, Biotechnology Research Institute, National Research Center, El-Buhouth St. 33, Dokki, P.O.12622, Giza, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundActinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial.

resultsIn this study, about 34 randomly selected Streptomyces isolates were discovered in soil, sediment, sea water, and other environments. Using a qualitative fast plate assay, they were tested for L-glutaminase production, and nine of them produced a significant amount of pink L-glutamine. Streptomyces sp. strain 5 M was identified by examining the 16S rRNA gene in the promising strain G8. A pH of 7.5, an incubation temperature of 40 °C, and the use of glucose and peptone as the carbon and nitrogen sources, respectively, produced the highest quantities of L-glutaminase. The molecular weight of the isolated L-glutaminase was estimated to be 52 kDa using SDS-PAGE analysis. At pH 7.5 and Temp., 40 °C, the isolated enzyme exhibited its highest levels of stability and activity. The isolated enzyme's K

conclusionOur findings include the discovery of Streptomyces sp. strain 5 M, which yields a free L-glutaminase and maybe a possible applicant for extra pharmacological investigation as an antineoplastic drug.

Indexed as

Antineoplastic AgentsGlutaminaseStreptomycesHeLa CellsHep G2 CellsHumansHydrogen-Ion ConcentrationMCF-7 CellsRNA, Ribosomal, 16SAntineoplastic AgentsGlutaminaseRNA, Ribosomal, 16SCytotoxicityL-glutaminaseMarine waterStrain 5 M.Streptomyces sp.

Identifiers

PMID39754133
PMCPMC11699688

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