ArticleBMC plant biology2025
Optimization of in vitro propagation and virus eradication using meristem culture and thermotherapy in two geranium species Pelargonium X hortorum ('Zonal') and Pelargonium × domesticum ('Regal').
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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- Conservation and valorization of Italian potato biodiversity through genotype-by-sequencing (GBS).Frontiers in plant science · 2025Article
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Abstract
backgroundGeraniums (Pelargonium) are among the most popular flowers worldwide. Viral infection is one of the main problems of the genus Pelargonium, and the production of virus-free mother plants is necessary for large-scale geranium propagation and exchange. Meristem culture and thermotherapy are two effective procedures that have been widely adopted to produce healthy virus-free plant stocks. The present study explores the efficiency of a combination of these two methods for virus eradication in two important Pelargonium species, Pelargonium X hortorum ('Zonal') and Pelargonium × domesticum ('Regal').
methodFor this purpose, RT-PCR have been performed using universal and specific primers of Tombusviridae and Bromoviridae virus families as well as Pelargonium Flower Break Virus (PFBV). Bud explants were taken from 'Zonal' and 'Regal' and were cultured in MS medium supplemented with different compositions of plant growth regulators (PGRs) as follow: A: (1 mgl
resultsPelargonium species exhibited varying responses to the PGR treatments. Specifically, the highest bud sprouting, plantlet regeneration, plantlet height, and root number were recorded in 'Zonal' and 'Regal' pelargoniums when cultured in media A and C, respectively. Although viral infection was confirmed in bud-derived plantlets using RT-PCR, thermotherapy and meristem culture resulted in the generation of 70% and 60% tombusviridae-free plantlets in 'Regal' and 'Zonal' Pelargoniums, respectively. The virus-free plantlets were propagated using the approved protocol.
conclusionThese findings underscore the significance of utilizing suitable PGRs for in vitro regeneration of each Pelargonium species. The results of this investigation revealed that RT-PCR using universal and specific primers is a reliable sensitive virus detection procedure that coupled with culturing the heat-treated meristem can result in successful viral eradication in Pelargonium species.
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