ArticleAnalytical chemistry2025
Decellularization and Enzymatic Digestion Methods to Enhance ECM Protein Detection via MALDI-MS Imaging.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Nascent extracellular matrix: the missing piece in hydrogel design.Cell biomaterials · 2026Article
- Applications and technical challenges of MALDI mass spectrometry imaging in microbial ecosystems and metabolic network research.Archives of microbiology · 2026Review
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Comparative proteomic analysis of the ECM composition of the human omentum and mesentery, the main sites of ovarian cancer metastasis.iScience · 2026Article
- MALDI mass spectrometry imaging of extracellular matrix proteins.The Analyst · 2025Review
- Protein lipidation in the tumor microenvironment: enzymology, signaling pathways, and therapeutics.Molecular cancer · 2025Review
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Authors and funding
7 authors.
Funding
Abstract
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) has been used to generate spatial maps of lipids, metabolites, peptides, proteins, and glycans in tissues; however, its use for mapping extracellular matrix (ECM) protein distributions is underexplored. ECM proteins play a major role in various pathological conditions, and changes in their spatial distributions affect the function and morphology of cells within tissues. ECM protein detection is challenging because they are large, insoluble, and undergo various post-translational modifications, such as glycosylation. We describe here decellularization of tissue sections coupled with serial enzymatic digestions with PNGaseF and trypsin to improve ECM protein detection in MALDI-MSI without disrupting ECM architecture. Decellularization leads to a 3-fold increase in the number of proteins that are measured by MALDI-MSI. We also introduce a binary colocalization method to improve protein identification, which increases the number of proteins that are confidently detected. Together, these methods enhance the spatial mapping of ECM proteins by MALDI-MSI.
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Registered trials
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