Evidence map›Paper›PMID 39752481›Full record

ArticleScience advances2025

High-resolution cryo-EM using a common LaB

Hariprasad Venugopal, Jesse Mobbs, Cyntia Taveneau, Daniel R Fox, Ziva Vuckovic, Sahil Gulati, Gavin Knott, Rhys Grinter, David Thal, Stephen Mick and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Thermionics in Topological Materials.Advanced materials (Deerfield Beach, Fla.) · 2025
    Review
  7. Extending the reach of single-particle cryoEM.Current opinion in structural biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hariprasad VenugopalRamaciotti Centre for Cryo-Electron Microscopy, Monash University, Clayton, VIC, Australia.ORCID 0000-0001-5230-2973
Jesse MobbsDrug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.ORCID 0000-0001-6979-1427
Cyntia TaveneauGlycoEra AG, Einsiedlerstrasse 34, 8820 Wädenswil, Switzerland.ORCID 0000-0002-3395-4957
Daniel R FoxAustralian Research Council Centre for Cryo-Electron Microscopy of Membrane Proteins, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.ORCID 0000-0003-0271-2776
Ziva VuckovicGlycoEra AG, Einsiedlerstrasse 34, 8820 Wädenswil, Switzerland.
Sahil GulatiGatan Inc., Pleasanton, CA, USA.ORCID 0000-0002-8255-479X
Gavin KnottDepartment of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia.ORCID 0000-0002-9007-6273
Rhys GrinterAustralian Research Council Centre for Cryo-Electron Microscopy of Membrane Proteins, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.
David ThalDrug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.ORCID 0000-0002-0325-2524
Stephen MickGatan Inc., Pleasanton, CA, USA.ORCID 0009-0007-6814-1255
Cory CzarnikGatan Inc., Pleasanton, CA, USA.ORCID 0009-0004-9528-3484
Georg RammRamaciotti Centre for Cryo-Electron Microscopy, Monash University, Clayton, VIC, Australia.ORCID 0000-0003-3596-2288

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-resolution cryo-electron microscopy (cryo-EM) requires costly 200- to 300-keV cryo-transmission electron microscopes (cryo-TEMs) with field emission gun (FEG) sources, stable columns, constant-powered lenses, autoloader, and direct electron detectors (DED). Recent advances in 100-keV imaging with the emergence of sub-200-keV optimized DED technology promises the development of more affordable cryo-TEMs. So far, 100-keV imaging has required microscopes with FEG sources. We here explored whether a standard 120-keV TEMs with thermionic lanthanum hexaboride (LaB

Identifiers

PMID39752481
PMCPMC11698077

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.