Evidence map›Paper›PMID 39752351›Full record

ArticlePloS one2025

miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway.

Han Zhao, Jiayu Zhang, Dengliang Li, Zhanding Cui, Jiuyuan Liu, Di Bao, Yiming Wei, Xinyue Zhang, Zhimin Wu, Tianqi Zhang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Han ZhaoCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Jiayu ZhangChangchun Chengkai Agricultural Investment Livestock Development Co.,Ltd.,Changchun, China.
Dengliang LiCollege of Agriculture, Tongren Polytechnic College, Tongren, Guizhou, Cnina.ORCID 0000-0002-1085-5294
Zhanding CuiDepartment of Veterinary Medicine, Key Laboratory of Preventive Veterinary Medicine, Animal Science College, Hebei North University, Zhangjiakou, Hebei, China.
Jiuyuan LiuCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Di BaoCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Yiming WeiCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Xinyue ZhangCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Zhimin WuSuzhou Jinweizhi Biotechnology Co.,Ltd, Suzhou, China.
Tianqi ZhangChengdu Zhentuo Pharmaceutical Technology Co., Ltd, Chendu, China.
Kai WangCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.
Shushuai YiCollege of Veterinary Medicine, Jilin Agricultural Science and Technology University, Jilin, China.
Wei LianJilin ZhengYe Biological Products Co.,Ltd., Jilin, China.
Guixue HuCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, China.ORCID 0009-0000-8473-3516

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) is a significant pathogen affecting swine, causing severe economic losses worldwide. This study explores the regulatory role of miRNA-328-3p to ZO-1 expression and its impact on PEDV proliferation via the PLC-β1-PKC pathway in IPEC-J2 cells. We found that miRNA-328-3p can target ZO-1, influencing its expression and subsequently affecting the integrity of tight junctions in the cells. Overexpression of PLC-β1, combined with miRNA-328-3p silencing, enhanced ZO-1 expression, while PLC-β1 knockdown combined with miRNA-328-3p overexpression inhibited ZO-1 expression. Furthermore, PLC-β1 overexpression increased both viral genome expression and PEDV titers, whereas its silencing had the opposite effect. Notably, our data indicated a negative correlation between PLC-β1 and PKC expression, and PKC silencing attenuated the upregulatory effect of PLC-β1 on ZO-1. These findings suggest that PLC-β1 modulates ZO-1 expression through the PKC pathway, providing new insights into the molecular mechanisms of PEDV infection and potential therapeutic targets.

Indexed as

MicroRNAsPhospholipase C betaPorcine epidemic diarrhea virusProtein Kinase CSignal TransductionZonula Occludens-1 ProteinAnimalsCell LineSwineVirus ReplicationMicroRNAsPhospholipase C betaProtein Kinase CZonula Occludens-1 Protein

Identifiers

PMID39752351
PMCPMC11698390

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.