Evidence map›Paper›PMID 39751919›Full record

ArticleMolecular biology reports2025

Mass-spectrometry-based quantitative proteomic analysis reveals that methylglyoxal and carnosine influence oxidative stress and RNA-processing associated proteins in renal proximal tubule epithelial cells.

Lei Liu, Shiqi Zhang, Juan Xu, Yadi Cao, Di Cui, Chao Liu, Bing Shen, Yonggui Wu, Qiu Zhang

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Lei Liu *Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Shiqi Zhang *Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Juan Xu *Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Yadi CaoDepartment of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Di CuiDepartment of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Chao LiuDepartment of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China.
Bing ShenSchool of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, People's Republic of China.
Yonggui Wu *Department of Nephrology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China. wuyonggui@medmail.com.cn.
Qiu Zhang *Department of Endocrinology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, People's Republic of China. zhangqiu@ahmu.edu.cn.

Funding

National Natural Science Foundation of China 81761138042National Natural Science Foundation of China 81900746
6 · The paper itself

Abstract

backgroundTubular injury triggered by hyperglycemia is an important pathological characteristic in diabetic nephropathy (DN). Accumulated advanced glycation end products and their precursor methylglyoxal (MGO), contribute to the development of DN. Carnosine has been shown to prevent the development of DN but the underlying mechanism still needs to be studied in depth. In this study, we explored the potential proteins influenced by MGO and carnosine in tubule epithelial cells. METHODS AND

resultsHK-2 cells were treated with MGO, carnosine, or a combination. Differentially expressed proteins (DEPs) between different groups were identified by isobaric tag for relative and absolute quantitation-based mass spectrometry. In the comparison between MGO and control, 29 DEPs were found to be associated with antioxidation and RNA methylation. In the comparison between carnosine and control, 10 DEPs were associated with ubiquitin protein ligase activity and RNA metabolism. In the comparison between MGO + carnosine and MGO, carnosine-induced DEPs in the presence of MGO were mainly related to RNA splicing and mRNA processing. MGO effects on OSTC expression was inversely correlated with that of carnosine. Some DEPs (OSTC, PRDX5, NEDD4L, NOP2, TRMT6, and GEMIN2) were validated by Western blotting. Additional experiments showed the 28 kD particle of Smith antigen was also influenced by MGO and carnosine.

conclusionsCarnosine can influence RNA processing and spliceosome-related proteins, and change MGO's effect on HK-2 cells. This study helps to understand the mechanism by which MGO contributes to the development of DN and promotes further identification of carnosine downstream proteins as therapeutic targets for DN.

Indexed as

CarnosineEpithelial CellsKidney Tubules, ProximalOxidative StressProteomicsPyruvaldehydeCell LineDiabetic NephropathiesHumansMass SpectrometryProteomeRNA Processing, Post-TranscriptionalCarnosineProteomePyruvaldehydeAlternative splicingCarnosineDiabetic nephropathyMethylglyoxalProteomics

Identifiers

PMID39751919

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