Evidence map›Paper›PMID 39749570›Full record

ArticleEmerging microbes & infections2025

Novel digital droplet inverse PCR assay shows that natural clearance of hepatitis B infection is associated with fewer viral integrations.

Dong Li, Vikki Ho, Chiao-Fang Teng, Hung-Wen Tsai, Yuanyuan Liu, Sarah Bae, Harout Ajoyan, Jochen M Wettengel, Ulrike Protzer, Brian S Gloss and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Emerging microbes & infections, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Dong LiStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Vikki HoStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Chiao-Fang TengGraduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
Hung-Wen TsaiDepartment of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
Yuanyuan LiuStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Sarah BaeStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Harout AjoyanStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Jochen M WettengelInstitute of Virology, Technische Universität München/Helmholtz Zentrum München, Munich, Germany.
Ulrike ProtzerInstitute of Virology, Technische Universität München/Helmholtz Zentrum München, Munich, Germany.
Brian S GlossScientific Platforms, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, Australia.
Rebecca J RockettCentre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, Westmead, NSW, Australia.
Rafid Al AsadyDepartment of Radiology, Westmead Hospital, Westmead, NSW, Australia.
Jane LiDepartment of Radiology, Westmead Hospital, Westmead, NSW, Australia.
Simon SoDepartment of Radiology, Westmead Hospital, Westmead, NSW, Australia.
Jacob GeorgeStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Mark W DouglasStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.
Thomas TuStorr Liver Centre, The Westmead Institute for Medical Research, Westmead Hospital and The University of Sydney, Westmead, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatitis B virus (HBV) DNA integration into the host cell genome is reportedly a major cause of liver cancer, and a source of hepatitis B surface antigen (HBsAg). High HBsAg levels can alter immune responses which therefore contributes to the progression of HBV-related disease. However, to what extent integration leads to the persistent circulating HBsAg is unclear. Here, we aimed to determine if the extent of HBV DNA integration is associated with the persistence of circulating HBsAg in people exposed to HBV. We established a digital droplet quantitative inverse PCR (dd-qinvPCR) method to quantify integrated HBV DNA in patients who had been exposed to HBV (anti-HBc positive and HBeAg-negative). Total DNA extracts from both liver resections (n = 32; 14 HBsAg-negative and 18 HBsAg-positive) and fine-needle aspirates (FNA, n = 10; 2 HBsAg-negative and 8 HBsAg-positive) were analysed. Using defined

Indexed as

Hepatitis BHepatitis B virusPolymerase Chain ReactionVirus IntegrationAdultAgedDNA, ViralFemaleHepatitis B Surface AntigensHumansLiverMaleMiddle AgedDNA, ViralHepatitis B Surface Antigensdigital droplet PCRfine needle aspirationfunctional cureHBsAgHBV DNA integration

Identifiers

PMID39749570
PMCPMC11731057

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.