Evidence map›Paper›PMID 39748275›Full record

ArticleBMC infectious diseases2025

Modified TPPA combined with western blotting facilitates syphilis diagnosis in isolated reactive treponemal chemiluminescence immunoassay sera: a prospective cohort study.

Xia Luo, Hua Xiao, Yanfang Lu, Weiming Gu, Ziyong Sun, Jing Peng, Liming Cheng

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Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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7 authors.

Xia Luo *Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hua Xiao *Department of Clinical Laboratory, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, 450007, Henan, People's Republic of China.
Yanfang LuDepartment of Integrated Traditional and Western Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Weiming GuShanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.
Ziyong SunDepartment of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Jing PengDepartment of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ppjj6@126.com.
Liming ChengDepartment of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. chengliming2015@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe challenge of dealing with isolated reactive treponemal chemiluminescence immunoassay (CIA) results in clinical practice has prompted the development of a more efficient algorithm for distinguishing true infection from false reactivity in isolated CIA sera.

methodsA prospective cohort study was conducted at Wuhan Tongji Hospital, involving 119,002 individuals screened for syphilis using CIA from January 1, 2015, to January 6, 2017. Samples with reactive CIA results underwent simultaneous testing with the T. pallidum passive particle agglutination assay (TPPA) and the rapid plasma reagin test (RPR). Additionally, a subgroup of 189 individuals with differing TPPA statuses was selected for further analysis using Western blotting (WB) and a modified TPPA assay (titer, 1:20). To identify the optimal serological approach for distinguishing true from false reactivity in sera with isolated reactive CIAs (CIA

resultsWB demonstrated lower sensitivity compared to TPPA, but it identified six syphilis cases among the 89 CIA

conclusionA combination of a modified TPPA (titer, 1:20) and WB can serve as a reliable algorithm for distinguishing true syphilis infection from false reactive signals in isolated reactive CIA sera. CLINICAL TRIAL NUMBER: Not applicable.

Indexed as

Blotting, WesternLuminescent MeasurementsSensitivity and SpecificitySyphilisSyphilis SerodiagnosisTreponema pallidumAdultAgglutination TestsAlgorithmsAntibodies, BacterialFemaleHumansImmunoassayMaleMiddle AgedProspective StudiesAntibodies, BacterialIsolated reactive chemiluminescence immunoassayModified TPPA (titer, 1:20)Syphilis serodiagnosisWestern blotting

Identifiers

PMID39748275
PMCPMC11697872

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.