Evidence map›Paper›PMID 39745653›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

CLADES: A Programmable Cascade of Genes for Cell Lineage Analysis and Manipulation.

Inés González-Aspe, Beatriz Madariaga Puchol, Blanca López Arrabal, Brooke Bosonac, Jorge García-Marqués

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Inés González-AspeInstituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Beatriz Madariaga PucholInstituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Blanca López ArrabalInstituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Brooke BosonacInstituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Jorge García-MarquésInstituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain. j.garcia.marques@csic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the Drosophila brain, neuronal diversity originates from approximately 100 neural stem cells, each dividing asymmetrically. Precise mapping of cell lineages at the single-cell resolution is crucial for understanding the mechanisms that direct neuronal specification. However, existing methods for high-resolution lineage tracing are notably time-consuming and labor-intensive. Here, we outline the best practices for lineage tracing using CLADES (cell lineage access driven by an edition sequence), a revolutionary approach to neuronal lineage tracing that addresses the limitations of previous methods. CLADES effectively traces the birth order of neurons using approximately 100 samples. The technique relies on a genetic cascade of reporter activations and deactivations that delineate lineage progression through color-coded markers. This system not only facilitates the detailed mapping of neuronal lineages but also holds the potential to be applied to tracking biological events and producing cell types for therapeutic purposes.

Indexed as

Cell LineageNeural Stem CellsAnimalsBrainCell DifferentiationDrosophilaGenes, ReporterNeuronsSingle-Cell AnalysisCaSSACRISPRDrosophilaGenetic cascadesGenetic toolsLineage tracingNeuronal specification

Identifiers

PMID39745653

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.