Evidence map›Paper›PMID 39745652›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Lineage Analysis at Single-Cell Resolution by Twin-Spot MARCM with Lineage-Restricted Drivers.

Angelica Previero, Ching-Po Yang, Tzumin Lee

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Angelica PrevieroLife Science Institute, University of Michigan, Ann Arbor, MI, USA.
Ching-Po YangLife Science Institute, University of Michigan, Ann Arbor, MI, USA.
Tzumin LeeLife Science Institute, University of Michigan, Ann Arbor, MI, USA. tzumin@umich.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell lineage analysis is primarily undertaken to understand cell fate specification and diversification along a cell lineage tree. Built with dual repressible markers, twin-spot mosaic analysis with repressible cell markers (MARCM) labels the two daughter cells made by a common precursor in distinct colors. The power of twin-spot MARCM to systematically subdivide complex lineages is exemplified in studies of Drosophila neural stem-cell lineages. To achieve targeted lineage analysis, lineage-restricted drivers can be created by restricting and immortalizing gene expression to a lineage of interest. Here, we describe detailed methods to perform lineage analysis in Drosophila neural stem-cell lineages utilizing lineage-specific drivers. Our innovative lineage tracing method can help resolve complex tissue development.

Indexed as

Cell LineageNeural Stem CellsSingle-Cell AnalysisAnimalsCell DifferentiationDrosophilaDrosophila melanogasterDrosophila ProteinsDrosophila ProteinsBinary sister fate decisionDrosophila brain developmentLineage specificityMosaic labelingNeuronal birth orderSingle-cell lineage reconstructionTwin-spot MARCM

Identifiers

PMID39745652

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.