Exploring Hox Genes and Their Temporal Expression in an Embryonic Model of Freshwater Crustaceans.

Michael L Jaramillo, Madson S Melo, Yara M R Müller, Dib Ammar, Evelise M Nazari

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Michael L JaramilloDepartamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

Madson S MeloDepartamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

Yara M R MüllerDepartamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

Dib AmmarDepartamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

Evelise M NazariDepartamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. evelise.nazari@ufsc.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hox genes have been investigated in various Arthropod species, resulting in the identification of ten Hox genes, organized in a colinear arrangement within the genome. Among arthropods, crustaceans exhibit a remarkable diversity of body shapes, which are associated with a variety of egg types, embryonic development patterns, and importantly, with the modulation of Hox genes to specify the identity of body segments along the antero-posterior axis of the embryo. Although there are more than 52,000 species of crustaceans described, their genomic resources are relatively limited, making it challenging to employ several molecular tools for studying embryonic development. In this regard, we present a protocol for identifying Hox genes in a freshwater prawn using degenerate primers and transcriptome analysis. This method enables the study of specific functions of Hox genes, thereby contributing to the evolutionary understanding of the diversity of body shapes in crustaceans.

Indexed as

CrustaceaGene Expression Regulation, DevelopmentalGenes, HomeoboxAnimalsEmbryonic DevelopmentEmbryo, NonmammalianFresh WaterGene Expression ProfilingTranscriptomeBody segmentationDegenerate primersEmbryonic developmentEmbryo transcriptomeEvo-devoMacrobrachium olfersii

Identifiers

PMID39745606

DOI10.3390/ijms17050690

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