Evidence map›Paper›PMID 39745451›Full record

ArticleJournal of virology2025

Discovery of small molecules against porcine reproductive and respiratory syndrome virus replication by targeting NendoU activity.

Jiaqi Zhu, Yunqiang Lai, Mengqi Cheng, Radha Charan Dash, Shuangshuang Guo, Jintong Guo, Yue Su, Andrew Wolek, Brianna Issacs, Zhenming Liu and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Jiaqi ZhuDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.ORCID 0000-0002-9066-5545
Yunqiang LaiShaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education and Universities of Shaanxi Province, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Mengqi ChengShaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education and Universities of Shaanxi Province, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Radha Charan DashDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut, USA.
Shuangshuang GuoDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.
Jintong GuoShaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education and Universities of Shaanxi Province, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Yue SuDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.
Andrew WolekDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.
Brianna IssacsDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.
Zhenming LiuState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Qi LiState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Neha MishraDepartment of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, Connecticut, USA.
Antonio GarmendiaDepartment of Pathobiology and Veterinary Sciences, University of Connecticut, Storrs, Connecticut, USA.
M Kyle HaddenDepartment of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut, USA.ORCID 0000-0001-9482-1712
X Cindy TianDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.ORCID 0000-0002-8441-2154
Xin HeShaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory of Livestock Biology, Engineering Research Center of Efficient New Vaccines for Animals, Ministry of Education and Universities of Shaanxi Province, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.ORCID 0000-0003-1579-3536
Young TangDepartment of Animal Science, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, USA.ORCID 0000-0001-6188-7758

Funding

U.S. Department of Agriculture (USDA) 2022-67016-37126
6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome (PRRS) remains a major threat to animal health and causes substantial economic losses worldwide. The nonstructural protein 11 (NSP11) of the causative agent, PRRS virus (PRRSV), contains a highly conserved nidoviral uridylate-specific endoribonuclease (NendoU) domain essential for viral replication and immune evasion. Targeting NSP11 offers a novel approach to antiviral intervention. Through IMPORTANCE: Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the pig industry, and vaccination is the principal method to prevent this viral infection currently. However, vaccination often fails to provide protection against heterologous strains, highlighting the need for alternative strategies for broad protection. The nidoviral uridylate-specific endoribonuclease (NendoU) domain plays a crucial role in viral replication and evasion of host immune responses. In this study, we identified a group of new compounds with similar chemical structures that could interfere with NendoU enzyme activity. Among these compounds, A8-A2 significantly inhibited PRRSV replication in host cells with minimal cytotoxicity. Our findings provide a new direction for developing potent antiviral compounds that can offer broad protection against different PRRSV strains, thereby mitigating their impact on pig health and benefiting the husbandry industry.

Indexed as

Antiviral AgentsEndoribonucleasesPorcine respiratory and reproductive syndrome virusViral Nonstructural ProteinsVirus ReplicationAnimalsCell LineDrug DiscoveryMacrophages, AlveolarMolecular Docking SimulationPorcine Reproductive and Respiratory SyndromeSwineAntiviral AgentsEndoribonucleasesnidoviral uridylate-specific endoribonucleaseViral Nonstructural Proteinsantiviral agentsNendoUnidovirusPRRSV

Identifiers

PMID39745451
PMCPMC11852993

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.