ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2025
Comparison of nasal swabs and handmade foam cubes for detecting equine herpesvirus 5 (EHV-5) by quantitative polymerase chain reaction (qPCR).
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
The control of equine respiratory infections is a biosecurity challenge. Respiratory viruses are often rapidly detected using quantitative polymerase chain reaction (qPCR) on nasal swabs. In the past, some laboratories developed handmade techniques to increase the amount of nasal secretions collected, without comparing them with nasal swabs when qPCR replaced the use of viral culture. The objectives of this study were to compare nasal swabs and handmade foam cubes for i) the detection of a common equine herpesvirus (EHV-5) by qPCR, and ii) their tolerability. Forty-five polyester swabs and foam cubes were used to sample 9 horses 5 times. These were then analyzed by qPCR for EHV-5. Agreement of qPCR results (positive, suspect, negative) was assessed using the intraclass correlation (ICC) and the avoidance scores were compared using a proportional odds mixed model. The ICC showed moderate agreement (0.61,
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39744469PMC11665725What OpenQuestion holds
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