Evidence map›Paper›PMID 39744342›Full record

ArticleCurrent medical mycology2024

Molecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods.

Ahmad Jabrodini, Mitra Zaighami, Ali Khodadadi, Keyvan Pakshir, Hasti Nouraei, Hossein Khodadadi

Abstract read
In one paragraph

Article in Current medical mycology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026
    Pooled it
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ahmad JabrodiniDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Mitra ZaighamiDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Ali KhodadadiFaculty of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, Isfahan, Iran.
Keyvan PakshirDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Hasti NouraeiDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Hossein KhodadadiDepartment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Purpose: Onychomycosis is a common fungal infection that affects the nails, caused by various fungal agents. Moreover, yeast onychomycosis has increased in recent years. Yeast isolates might not be identified at the species level by conventional methods, whereas molecular methods can identify yeast isolates more accurately. This study aimed to identify yeast communities isolated from nail specimens by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and PCR- fragment size polymorphism (FSP) methods. Materials and Methods: This experimental study was conducted on archival yeast isolates obtained from 269 patients suspected of onychomycosis who referred to the Medical Mycology Laboratory at Shiraz University of Medical Sciences in Shiraz, Iran, between April 2022 and March 2023. Onychomycosis was confirmed through direct examination and culture of nail specimens. The PCR-RFLP and PCR-FSP methods were used to identify yeast isolates. Results: In total, 78 (28.99%) yeast strains were identified. Conclusion: It seems necessary to use molecular diagnostic tools in addition to conventional methods to identify yeast isolates in clinical laboratories. The rapid and accurate identification of fungal agents causing onychomycosis is useful for the selection of an appropriate treatment strategy.

Indexed as

Molecular identificationOnychomycosisPCR-FSPPCR-RFLPYeast communities

Identifiers

PMID39744342
PMCPMC11688582

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.