Evidence map›Paper›PMID 39744094›Full record

ArticlemLife2024

Measurement of optical density of microbes by multi-light path transmission method.

Hongwei Wang, Carina M Gu, Sujuan Xu, Hongfeng Wang, Xiaomin Zhao, Lichuan Gu

Abstract read
In one paragraph

Article in mLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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  5. Article
  6. Optimizing chromosome dispersion quality: the key role of cell density.Frontiers in cell and developmental biology · 2025
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hongwei WangState Key Laboratory of Microbial Technology Shandong University Qingdao China.
Carina M GuState Key Laboratory of Microbial Technology Shandong University Qingdao China.
Sujuan XuState Key Laboratory of Microbial Technology Shandong University Qingdao China.
Hongfeng WangShandong Peanut Research Institute Qingdao China.
Xiaomin ZhaoState Key Laboratory of Microbial Technology Shandong University Qingdao China.
Lichuan GuState Key Laboratory of Microbial Technology Shandong University Qingdao China.ORCID 0000-0002-8201-403X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optical density (OD) is an important indicator of microbial density, and a commonly used variable in growth curves to express the growth of microbial culture. However, OD values show a linear relationship with bacterial concentration only at low concentrations. When the cell density is high, the relationship loses linearity, and serial dilution is needed to obtain readings of better accuracy. Here, we show that measuring OD values using shorter light paths is in close equivalence to measuring OD values of the cell culture with corresponding dilution. By measuring three different light paths simultaneously, accurate OD values can be easily obtained from low to high cell density. Using this method, growth curves of

Indexed as

Beer–Lambert lawcell densitygrowth curvemicrobeoptical density

Identifiers

PMID39744094
PMCPMC11686084

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.