Evidence map›Paper›PMID 39741253›Full record

ArticleBMC oral health2024

Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement.

Liyuan Chen, Danqing He, Zixin Li, Shengjie Cui, Min Yu, Zimo Zhao, Yuetong Chen, Jiayi Song, Nan Jiang, Huajie Yu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Liyuan Chen *Department of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Danqing He *Department of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Zixin LiDepartment of Stomatology, Peking University People's Hospital, Beijing, China.
Shengjie CuiDepartment of General Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Min YuDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Zimo ZhaoDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Yuetong ChenDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Jiayi SongDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China.
Nan JiangCentral Laboratory, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.
Huajie YuPeking University Hospital of Stomatology Fourth Division, No.41 Dongsihuan Zhong Road, Beijing, China. yuhuajie666@163.com.
Yan LiuDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School, 22th Zhongguancun South Ave, Beijing, 100081, China. orthoyan@bjmu.edu.cn.

Funding

Angelalign Scientific Research Fund EARD20220725046Innovative Research Team of High-level Local Universities in Shanghai SHSMU-ZLCX20212402Key R & D Plan of Ningxia Hui Autonomous Region 2020BCG01001Peking University Clinical Medicine Plus X - Young Scholars Project PKU2023LCXQ004the Beijing International Science and Technology Cooperation Project Z221100002722003the Beijing Natural Science Foundation 7242282the Beijing Natural Science Foundation J230032the Beijing Natural Science Foundation L234017the National Natural Science Foundations of China 82101043the National Natural Science Foundations of China 82170996the National Natural Science Foundations of China 82230030
6 · The paper itself

Abstract

backgroundOrthodontic tooth movement (OTM) relies on the remodeling of periodontal tissues, including the periodontal ligament (PDL) and alveolar bone. Collagen remodeling plays a crucial role during this process, allowing for the necessary changes in the PDL's structure and function. Endo180, an urokinase plasminogen activator receptor-associated protein, is a transmembrane receptor regulated collagen remodeling. This study aims to investigate whether and how Endo180 participates in collagen remodeling within the PDL during OTM. MATERIALS AND

methodsA mechanical force-induced OTM rat model was established using a closed coiled spring to mesially move the right maxillary first molar. The distance of OTM was examined by micro-computed tomography (micro-CT). The collagen remodeling within the PDL was assessed using atomic force microscope (AFM), Hematoxylin-Eosin (HE) staining and Masson staining. Protein expressions of Endo180, collagen I (COL I) and collagen III (COL III) were analyzed via immunofluorescence staining. Additionally, the mRNA expressions of Endo180, COL I, and COL III in force-induced PDL cells were examined by RT-qPCR in vitro. To further illustrate the role of Endo180 in regulating COL I and COL III expressions, Endo180 siRNA (siEndo) was applied to force-stimulated PDL cells.

resultsForce application increased OTM distance and disrupted collagen fiber organization, with a greater decrease in collagen elastic modulus on the mesial side than on the distal side of the PDL. After 7 days of force application, Endo180 and COL III expressions significantly increased in PDL tissues, while COL I expression decreased in PDL tissues. Compressive force loading in vitro upregulated the mRNA expressions of Endo180 and COL III, but downregulated COL I mRNA expression. Notably, Endo180 knockdown using siRNA suppressed force-induced COL III expression while restoring the downregulated COL I expression under compressive force stimuli.

conclusionForce-induced Endo180 expression modulates collagen remodeling in PDL during OTM by upregulating COL III and downregulating COL I. This collagen reorganization facilitates efficient tooth movement, highlighting Endo180 as a potential therapeutic target to optimize orthodontic treatment outcomes.

Indexed as

Periodontal LigamentTooth Movement TechniquesX-Ray MicrotomographyAnimalsCollagenCollagen Type ICollagen Type IIIMaleMicroscopy, Atomic ForceRatsRats, Sprague-DawleyCollagenCollagen Type ICollagen Type IIICollagen remodelingEndo180Mechanical forceOrthodontic tooth movementPeriodontal ligament cell

Identifiers

PMID39741253
PMCPMC11689597

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.