ArticleScientific reports2024
Improving small-scale cultivation of Spodoptera frugiperda 9 cells by silanizing glassware.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Effects of antifoam agents on Spodoptera frugiperda 9 cell growth and baculovirus infection dynamics.Journal of biological engineering · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultivating insect cells in glass vessels can be challenging. Due to uncontrolled cell adhesion and associated cell loss as well as clumping, the replication of experiments is put at risk. A cost-effective solution to improve and stabilize cultivation may be to silanize glass vessels, making them more hydrophobic and chemically inert. Therefore, the cell growth parameters of Spodoptera frugiperda (Sf) 9 cells were characterized in an experiment comparing silanized and non-silanized vessels of three different sizes. Silanization had a significantly positive effect on living cell count and viability in small-scale cultivation (10 ml culture tubes and 50 ml culture flasks) by reducing adhesion of cells to the vessel walls. The treatment also improved the comparability of the biological replicates by reducing the variance of measurements. No such effects could be observed for larger 250 ml culture flasks. Overall, the results indicate that silanization can improve cultivation, especially when working with small glass vessels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.