Evidence map›Paper›PMID 39741008›Full record

ArticleLife science alliance2025

Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification.

Jiaxi Cai, Yun Quan, Cindy Yuxuan Zhang, Ziyi Wang, Stephen M Hinshaw, Huilin Zhou, Raymond T Suhandynata

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life science alliance, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Jiaxi Caihttps://ror.org/0168r3w48 Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, USA.ORCID 0000-0002-4487-5613
Yun Quanhttps://ror.org/0168r3w48 Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, USA.ORCID 0000-0003-3230-6189
Cindy Yuxuan Zhanghttps://ror.org/0168r3w48 Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, USA.
Ziyi Wanghttps://ror.org/0168r3w48 Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, USA.ORCID 0009-0008-2961-3173
Stephen M Hinshawhttps://ror.org/00f54p054 Department of Chemical and Systems Biology, Stanford University, Palo Alto, CA, USA.ORCID 0000-0003-4215-5206
Huilin Zhouhttps://ror.org/0168r3w48 Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, USA huzhou@health.ucsd.edu.ORCID 0000-0002-1350-4430
Raymond T Suhandynatahttps://ror.org/0168r3w48 Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, San Diego, CA, USA rtsuhandynata@health.ucsd.edu.ORCID 0000-0002-4767-7639

Funding

Suppression of duplication-mediated genome rearrangements by protein sumoylationR01GM116897 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHOU, HUILIN · 2015 to 2023
$2.6M
Kinetochore Assembly and RegulationR01GM151191 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Arshad Desai, Raymond Theodore Suhandynata · 2023 to 2026
$1.8M
Orbitrap Fusion Mass SpectrometerS10OD023498 · OD · LUDWIG INSTITUTE FOR CANCER RES LTD · PI ZHOU, HUILIN · 2017 to 2017
$600k
NIGMS NIH HHS R01 GM116897NIGMS NIH HHS R01 GM151191NIH HHS S10 OD023498
6 · The paper itself

Abstract

Large multiprotein machines are central to many biological processes. However, stoichiometric determination of protein complex subunits in their native states presents a significant challenge. This study addresses the limitations of current tools in accuracy and precision by introducing concatemer-assisted stoichiometry analysis (CASA). CASA leverages stable isotope-labeled concatemers and liquid chromatography-parallel reaction monitoring-mass spectrometry (LC-PRM-MS) to achieve robust quantification of proteins with sub-femtomole sensitivity. As a proof of concept, CASA was applied to study budding yeast kinetochores. Stoichiometries were determined for ex vivo reconstituted kinetochore components, including the canonical H3 nucleosomes, centromeric (Cse4

Indexed as

KinetochoresMass SpectrometrySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCentromereChromatography, LiquidChromosomal Proteins, Non-HistoneNucleosomesProteomicsChromosomal Proteins, Non-HistoneNucleosomesSaccharomyces cerevisiae Proteins

Identifiers

PMID39741008
PMCPMC11707388

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.