Evidence map›Paper›PMID 39739530›Full record

ArticleCell reports2025

mRNA decay pre-complex assembly drives timely cell-state transitions during differentiation.

Hideyuki Komori, Geeta Rastogi, John Paul Bugay, Hua Luo, Sichun Lin, Stephane Angers, Craig A Smibert, Howard D Lipshitz, Cheng-Yu Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. RNA-binding is the essential biological function of thebioRxiv : the preprint server for biology · 2026
    Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Hideyuki KomoriLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Geeta RastogiLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
John Paul BugayDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Hua LuoDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5G 1M1, Canada.
Sichun LinDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.
Stephane AngersDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5G 1M1, Canada.
Craig A SmibertDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5G 1M1, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5G 1M1, Canada.
Howard D LipshitzDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5G 1M1, Canada.
Cheng-Yu LeeLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Division of Genetic Medicine, Department of Internal Medicine and Rogel Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: leecheng@umich.edu.

Funding

Shared mechanisms regulate transcription-factor activity to control cell fate in neural stem cells and the embryoR01NS111647 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI HARRISON, MELISSA, LEE, CHENG-YU · 2019 to 2023
$1.7M
Multi-layered Control of the Exit from StemnessR01NS107496 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LEE, CHENG-YU · 2019 to 2023
$1.7M
Regulation of generation and functions of intermediate progenitorsR01NS134942 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Cheng-Yu Lee · 2024 to 2026
$1.3M
NINDS NIH HHS R01 NS107496NINDS NIH HHS R01 NS111647NINDS NIH HHS R01 NS134942
6 · The paper itself

Abstract

Complexes that control mRNA stability and translation promote timely cell-state transitions during differentiation by ensuring appropriate expression patterns of key developmental regulators. The Drosophila RNA-binding protein brain tumor (Brat) promotes the degradation of target transcripts during the maternal-to-zygotic transition in syncytial embryos and uncommitted intermediate neural progenitors (immature INPs). We identify ubiquitin-specific protease 5 (Usp5) as a candidate Brat interactor essential for the degradation of Brat target mRNAs. Usp5 promotes the formation of the Brat-deadenylase pre-complex in mitotic neural stem cells (neuroblasts) by facilitating Brat interactions with the scaffolding components of deadenylase complexes. The adaptor protein Miranda binds the RNA-binding domain of Brat, limiting its ability to bind target mRNAs in mitotic neuroblasts. Cortical displacement of Miranda activates Brat-deadenylase complex activity in immature INPs. We propose that the assembly of an enzymatically inactive and RNA-binding-deficient pre-complex poises mRNA degradation machineries for rapid activation, driving timely developmental transitions.

Indexed as

Cell DifferentiationDrosophila melanogasterDrosophila ProteinsRNA, MessengerRNA StabilityAnimalsDNA-Binding ProteinsNeural Stem CellsRNA-Binding ProteinsUbiquitin-Specific Proteasesbrat protein, DrosophilaDNA-Binding ProteinsDrosophila ProteinsRNA-Binding ProteinsRNA, MessengerUbiquitin-Specific ProteasesBratcell state transitionsCP: Developmental biologyCP: Molecular biologydeadenylase complexesdifferentiationDrosophilamaternal mRNAsmRNA decayneuroblastUsp5

Identifiers

PMID39739530
PMCPMC11911916

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.