Evidence map›Paper›PMID 39739380›Full record

ArticleBiomolecules & biomedicine2025

N6-methyladenosine methylation regulators can serve as potential biomarkers for endometriosis related infertility.

Yalun He, Jie Ding, Tonglin Bai, Yangshuo Li, Xiaolan Liang, Yiming Chen, Yi Lin, Wen Cheng, Chaoqin Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules & biomedicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yalun HeDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China; Department of Physiotherapy of Traditional Chinese Medicine, Beidaihe Rehabilitation and Recuperation Center, The People's Liberation Army Joint Logistic Support Force, Qinhuangdao, China.
Jie DingDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Tonglin BaiDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yangshuo LiDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Xiaolan LiangDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yiming ChenDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.
Yi LinDepartment of Reproductive Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Wen ChengDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China; Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Chaoqin YuDepartment of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endometriosis (EMS) is a chronic inflammatory disease frequently associated with infertility. N6-methyladenosine (m6A) methylation, the most common form of methylation in eukaryotic mRNAs, has gained attention in the study of female reproductive diseases, including EMS and infertility. This study aimed to investigate the role of m6A regulators in EMS-related infertility. To begin, specific m6A regulators were identified by analyzing the GSE120103 dataset, followed by receiver operating characteristic (ROC) curve analysis. A nomogram model was then constructed, and unsupervised clustering of m6A regulators was performed to identify distinct m6A molecular clusters. Functional enrichment analysis of differentially expressed genes (DEGs) between these clusters, along with immune cell infiltration analysis, was subsequently conducted. In addition, the single-cell dataset GSE214411 was analyzed to explore the role of m6A regulators in various cell types. Finally, clinical samples were collected, and immunohistochemistry analysis was performed. The study identified seven key m6A regulators with significant diagnostic value for EMS-related infertility and two distinct m6A molecular clusters. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs between the clusters revealed that m6A clustering was strongly associated with immune pathways. Immune cell infiltration analysis further demonstrated that the expression levels of m6A regulators had a notable impact on immune cell infiltration. Single-cell analysis revealed that HNRNPA2B1 and HNRNPC were significantly elevated in endometrial immune cells from infertile EMS patients but notably decreased in stromal cells. Immunohistochemical staining confirmed that HNRNPA2B1 and HNRNPC expression levels were significantly higher in the eutopic endometrium of fertile women compared to ovarian EMS patients. These findings suggest that m6A regulators play critical roles in the development and progression of EMS-related infertility. Notably, HNRNPA2B1 and HNRNPC may serve as potential biomarkers for this condition.

Indexed as

AdenosineEndometriosisInfertility, FemaleAdultBiomarkersFemaleGene Expression ProfilingHumansMethylationAdenosineBiomarkersN-methyladenosine

Identifiers

PMID39739380
PMCPMC12097398

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