Evidence map›Paper›PMID 39738760›Full record

ArticleScientific reports2024

Impact of land-use and fecal contamination on Escherichia populations in environmental samples.

Adrian L Cookson, Jonathan C Marshall, Patrick J Biggs, Lynn E Rogers, Rose M Collis, Megan Devane, Rebecca Stott, Gale Brightwell

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. [Evaluation of Gradient-Variant Quantum Dot Fluorescence Technology for Rapid Detection ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Adrian L CooksonFood System Integrity, AgResearch Limited, Hopkirk Research Institute, Massey University, Cnr University Avenue and Library Road, Private Bag 11008, Palmerston North, 4442, New Zealand. adrian.cookson@agresearch.co.nz.
Jonathan C MarshallmEpiLab, School of Veterinary Science, Massey University, Palmerston North, New Zealand.
Patrick J BiggsmEpiLab, School of Veterinary Science, Massey University, Palmerston North, New Zealand.
Lynn E RogersFood System Integrity, AgResearch Limited, Hopkirk Research Institute, Massey University, Cnr University Avenue and Library Road, Private Bag 11008, Palmerston North, 4442, New Zealand.
Rose M CollisFood System Integrity, AgResearch Limited, Hopkirk Research Institute, Massey University, Cnr University Avenue and Library Road, Private Bag 11008, Palmerston North, 4442, New Zealand.
Megan DevaneEnvironmental Science and Research Limited, Christchurch, New Zealand.
Rebecca StottNational Institute of Water and Atmospheric Research, Hamilton, New Zealand.
Gale BrightwellFood System Integrity, AgResearch Limited, Hopkirk Research Institute, Massey University, Cnr University Avenue and Library Road, Private Bag 11008, Palmerston North, 4442, New Zealand.

Funding

Ministry of Business, Innovation and Employment C10X1507
6 · The paper itself

Abstract

Understanding the composition of complex Escherichia coli populations from the environment is necessary for identifying strategies to reduce the impacts of fecal contamination and protect public health. Metabarcoding targeting the hypervariable gene gnd was used to reveal the complex population diversity of E. coli and phenotypically indistinct Escherichia species in water, soil, sediment, aquatic biofilm, and fecal samples from native forest and pastoral sites. The resulting amplicons were cross-referenced against a database containing over 700 different partial gnd sequences from E. coli/non-E. coli Escherichia species. Alpha and beta measures of diversity of Escherichia populations were lowest in feces, soil and sediment compared to water and aquatic biofilm samples. Escherichia populations recovered from extensive freshwater catchments dominated by sheep, beef and dairy farming were extremely diverse but well-separated from a wetland dairy site. In contrast, Escherichia populations from the low-impact native forest site with fewer fecal sources were less diverse. Metabarcoding distinguished E. coli populations important to fecal contamination monitoring from non-E. coli Escherichia environmental populations. These data represent in-depth analysis and geographic stability of Escherichia populations from environmental samples with extensive heterogeneity, and reveal links with diverse fecal sources, land-use and the overall burden of fecal contamination at sample sites.

Indexed as

Escherichia coliFecesAnimalsDNA Barcoding, TaxonomicEnvironmental MonitoringSoil MicrobiologyWater MicrobiologyFecal indicator bacteriaFreshwaterLand-useMicrobial community profilingSource trackingZoonotic pathogens

Identifiers

PMID39738760
PMCPMC11685573

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.