Evidence map›Paper›PMID 39738271›Full record

ArticleScientific reports2024

Jararaca GPIb-binding protein causes thrombocytopenia during Bothrops jararaca envenomation.

Marcelo Larami Santoro, Ana Teresa Azevedo Sachetto, Jaqueline Gomes Rosa, Ricardo José Soares Torquato, Débora Andrade-Silva, Dilza Trevisan-Silva, Cynthia Zaccanini de Albuquerque, Solange M T Serrano, Vânia Gomes de Moura Mattaraia, Aparecida Sadae Tanaka and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marcelo Larami SantoroBiotério Central, Instituto Butantan, Av. Dr. Vital Brasil, 1500, São Paulo, SP, 05503 - 900, Brazil. marcelo.santoro@butantan.gov.br.
Ana Teresa Azevedo SachettoBiotério Central, Instituto Butantan, Av. Dr. Vital Brasil, 1500, São Paulo, SP, 05503 - 900, Brazil.
Jaqueline Gomes RosaBiotério Central, Instituto Butantan, Av. Dr. Vital Brasil, 1500, São Paulo, SP, 05503 - 900, Brazil.
Ricardo José Soares TorquatoDepartmento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Débora Andrade-SilvaLaboratório de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil.
Dilza Trevisan-SilvaLaboratório de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil.
Cynthia Zaccanini de AlbuquerqueBiotério Central, Instituto Butantan, Av. Dr. Vital Brasil, 1500, São Paulo, SP, 05503 - 900, Brazil.
Solange M T SerranoLaboratório de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil.
Vânia Gomes de Moura MattaraiaBiotério Central, Instituto Butantan, Av. Dr. Vital Brasil, 1500, São Paulo, SP, 05503 - 900, Brazil.
Aparecida Sadae TanakaDepartmento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Maria Elisa PeichotoConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

Funding

Agencia I+D+i PICT-2020-SERIEA-02027CONICET/FAPESP 2016Conselho Nacional de Desenvolvimento Científico e Tecnológico 302703/2017-9Conselho Nacional de Desenvolvimento Científico e Tecnológico 312469/2018-7Fundação Butantan 001/0708/000.223/2024Fundação de Amparo à Pesquisa do Estado de São Paulo 2012/03657-8Fundação de Amparo à Pesquisa do Estado de São Paulo 2013/07467-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/16935-7Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/50411-5Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/00715-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/26015-8Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/07618-6
6 · The paper itself

Abstract

Inoculation of Bothrops jararaca snake venom (BjV) induces thrombocytopenia in humans and various animal species. Although several BjV toxins acting on hemostasis have been well characterized in vitro, it is not known which one is responsible for inducing thrombocytopenia in vivo. In previous studies, we showed that BjV incubated with metalloproteinase or serine proteinase inhibitors and/or anti-botrocetin antibodies still induced thrombocytopenia in rats and mice. Thus, herein we identified and characterized BjV toxins responsible for inducing thrombocytopenia. Initially, by filtering BjV on ultrafiltration systems, proteins with molecular masses between 30 and 50 kDa were shown to induce thrombocytopenia in mice, but they were not associated with hemorrhagic or coagulating activities. The 50 kDa ultrafiltrate was chromatographed, and two proteins (named fraction D and fraction E) induced thrombocytopenia in mice. However, neither fraction D nor fraction E induced platelet aggregation in platelet-rich plasma or whole blood from humans or mice. By mass spectrometry analysis, fraction E was identified as jararaca glycoprotein Ib (GPIb)-binding protein. Injection of these fractions caused thrombocytopenia in control or Vwf

Indexed as

BothropsThrombocytopeniaAnimalsBothrops jararacaBothrops jararaca VenomCrotalid VenomsFemaleHumansMaleMiceMice, KnockoutPlatelet AggregationPlatelet Glycoprotein GPIb-IX ComplexSnake Bitesvon Willebrand FactorBothrops jararaca VenomCrotalid VenomsPlatelet Glycoprotein GPIb-IX Complexvon Willebrand FactorBleedingBlood plateletsC-type lectin-like proteinsFibrinogenGlycoprotein Ib-binding proteinSnaclecThrombocytopenia

Identifiers

PMID39738271
PMCPMC11686094

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.