Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective.

Introduction: Clinical metritis (CM) has significant costs to dairy producers. Current treatment strategy involves systemic antibiotics; however, there is increasing concern about judicious antibiotic use. The study objective was to evaluate the effects of a non-antibiotic treatment vs. systemic antibiotic therapy on the vaginal discharge microbiome of dairy cows diagnosed with CM at 7 ± 3 DIM (days in milk). We hypothesize that both treatment methods will have a similar impact on the reproductive microbiome due to broad-spectrum bactericidal activity; therefore, there will not be significant differences amongst the microbiota after the completion of therapy. Methods: Cows from a central Pennsylvania dairy were screened for CM at 7 ± 3 days DIM using a Metricheck™ device ( Results: After treatment, there were only minor differences within the microbiome between the two treatments indicating the potential suitability of dextrose as an antibiotic-alternative treatment. Alpha diversity did not differ (Welch's Discussion: While 16S rRNA analysis does not provide information on bacterial viability, the relative similarity of the microbiome between the two groups immediately following treatment might suggest that intrauterine dextrose could be utilized as an alternative treatment for CM.