Evidence map›Paper›PMID 39736772›Full record

ArticleBMC research notes2024

Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae).

Ha Yeun Song, Young Ji Choi, Dea-Sung Lee, Hyun Ki Choi, Kyung Min Choi, Kang-Yeol Yu, Namki Cho, Seungki Lee

Abstract read
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Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Ha Yeun SongHonam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.
Young Ji ChoiHonam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.
Dea-Sung LeeNational Marine Biodiversity Institute of Korea, Seochun, 33662, Republic of Korea.
Hyun Ki ChoiNational Institute of Biological Resources, Inchen, 22689, Republic of Korea.
Kyung Min ChoiHonam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.
Kang-Yeol YuHonam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.
Namki ChoCollege of Pharmacy, Chonnam National University, Gwangju, 61186, Republic of Korea.
Seungki LeeNational Institute of Biological Resources, Inchen, 22689, Republic of Korea. metany@korea.kr.

Funding

project to make multi-ministerial national biological research resources more advanced program RS-2023-00230404
6 · The paper itself

Abstract

objectiveThe ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing.

resultsOut of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species.

Indexed as

Microsatellite RepeatsAllelesAnimalsBrachyuraGenetic LociHeterozygoteHigh-Throughput Nucleotide SequencingPolymorphism, GeneticRepublic of KoreaConservationGenetic variabilityMicrosatellite lociOcypode stimpsoni

Identifiers

PMID39736772
PMCPMC11684255

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