ArticleBMC plant biology2024
Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.
Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing.Journal, genetic engineering & biotechnology · 2026Article
- Red and Far-Red LED Lighting Enhances Protoplast-to-Plant Regeneration in Broccoli (Plants (Basel, Switzerland) · 2026Article
- In Vitro Micropropagation of Oca (Plants (Basel, Switzerland) · 2025Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- Nutritional, Therapeutic, and Functional Food Perspectives of Kale (Molecules (Basel, Switzerland) · 2025Review
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
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Authors and funding
4 authors.
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Abstract
backgroundBrassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B. oleracea var. viridis) is a non-heading leafy vegetable grown in urban farms and community gardens in the United States and Europe. Improving collard and other Brassica germplasm can benefit from both traditional and new plant breeding technologies (NPBTs), such as CRISPR-Cas mediated transformation. An efficient transformation or protoplast fusion can only be achieved with a robust and reproducible protocol for protoplast-to-plant regeneration. This research focuses on optimizing in vitro culture conditions to enhance cell divisions, microcallus formation, and the regeneration of shoots and roots in four Brassica oleracea varieties, including collard.
resultsThe protocol of protoplast release, purification and immobilization was optimized to obtain a suitable number and quality of protoplasts from seven cultivars of B. oleracea. The protoplast isolation efficiency after digestion of young leaves in optimized enzyme solution reached on average 2.5 × 10
conclusionsThe results of this research have led to the development of a robust protoplast-to-plant regeneration protocol for four varieties of B. oleracea that could be exploited as a tool for production of transformants and somatic hybrids. Furthermore, we present the first successful regeneration of protoplast-derived plants of collard, an overlooked but valuable variety of Brassica oleracea.
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