Evidence map›Paper›PMID 39734219›Full record

ArticleMicrobial cell factories2024

A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440.

Qifeng Wen, JinJin Chen, Jin Li, Ida Putu Wiweka Dharmasiddhi, Maohua Yang, Jianmin Xing, Yilan Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Development of genetic manipulation tools forFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qifeng WenState Key Laboratory of Petroleum Molecular & Process Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
JinJin ChenDepartment of Chemical Engineering, University of Waterloo, Waterloo, Canada.
Jin LiDepartment of Chemical Engineering, University of Waterloo, Waterloo, Canada.
Ida Putu Wiweka DharmasiddhiDepartment of Chemical Engineering, University of Waterloo, Waterloo, Canada.
Maohua YangState Key Laboratory of Petroleum Molecular & Process Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China. mhyang@ipe.ac.cn.
Jianmin XingState Key Laboratory of Petroleum Molecular & Process Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China. jmxing@ipe.ac.cn.
Yilan LiuDepartment of Chemical Engineering, University of Waterloo, Waterloo, Canada. yilan.liu@uwaterloo.ca.

Funding

National Key Research and Development Program of China 2023YFC3403601
6 · The paper itself

Abstract

backgroundPseudomonas putida KT2440, a non-pathogenic soil bacterium, is a key platform strain in synthetic biology and industrial applications due to its robustness and metabolic versatility. Various systems have been developed for genome editing in P. putida, including transposon modules, integrative plasmids, recombineering systems, and CRISPR/Cas systems. However, rapid iterative genome editing is limited by complex and lengthy processes.

resultsWe discovered that the pBBR1MCS2 plasmid carrying the CRISPR/Cas9 module could be easily cured in P. putida KT2440 at 30

conclusionsWe developed and applied a rapid all-in-one plasmid CRISPR/Cas9 system for genome editing in P. putida. This system requires less than 1.5 days for one edit due to simplified plasmid construction, electroporation and curing processes, thus accelerating the cycle of genome editing. To our knowledge, this is the fastest iterative genome editing system for P. putida. Using this system, we rapidly engineered P. putida for valencene biosynthesis for the first time, showcasing the system's potential for expanding biotechnological applications.

Indexed as

CRISPR-Cas SystemsGene EditingPlasmidsPseudomonas putidaGenome, BacterialAll-in-one CRISPR/Cas9 systemBiosynthesisGenome editingPlasmid curingPseudomonas putida KT2440

Identifiers

PMID39734219
PMCPMC11684315

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.