Evidence map›Paper›PMID 39733129›Full record

ArticleScientific reports2024

Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein.

Adriano E Pereira, Kyle J Paddock, Jacob A Corcoran, Zixiao Zhao, Michelle L J Gregory, Thomas A Coudron, Bruce E Hibbard, Kent S Shelby, Man P Huynh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Adriano E PereiraDivision of Plant Science and Technology, University of Missouri, Columbia, MO, 65211, USA.
Kyle J PaddockBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Jacob A CorcoranBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Zixiao ZhaoDivision of Plant Science and Technology, University of Missouri, Columbia, MO, 65211, USA.
Michelle L J GregoryBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Thomas A CoudronBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Bruce E HibbardBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Kent S ShelbyBiological Control of Insects Research Laboratory, USDA-ARS, Columbia, MO, 65203, USA.
Man P HuynhDivision of Plant Science and Technology, University of Missouri, Columbia, MO, 65211, USA. manhuynh@missouri.edu.

Funding

Syngenta Biotechnology 58-3K95-4-1697
6 · The paper itself

Abstract

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, has evolved resistance to nearly every management tactic utilized in the field. This study investigated the resistance mechanisms in a WCR strain resistant to the Bacillus thuringiensis (Bt) protein eCry3.1Ab using dsRNA to knockdown WCR midgut genes previously documented to be associated with the resistance. ATP-binding cassette transporter (ABCC4), aminopeptidase-N, cadherin, and cathepsin-B were previously found to be differentially expressed in eCry3.1Ab-resistant WCR larvae when compared to susceptible larvae after feeding on maize expressing eCry3.1Ab and its near-isoline. Here we compared the susceptibility of resistant and susceptible WCR larvae to eCry3.1Ab protein in presence or absence of dsRNA targeting the above genes using 10-day diet overlay toxicity assays. Combining ABCC4 dsRNA with eCry3.1Ab protein increased susceptibility to Bt protein in WCR-resistant larvae, but the other three genes had no such effect. Among 65 ABC transport genes identified, several were expressed differently in resistant or susceptible WCR larvae, fed on eCry3.1Ab-expressing maize versus its isoline, that may be involved in Bt resistance. Our findings provide strong evidence that ABCC4 is indirectly involved in WCR resistance to eCry3.1Ab protein by enhancing the effects of Bt-induced toxicity.

Indexed as

ATP-Binding Cassette TransportersBacillus thuringiensis ToxinsBacterial ProteinsColeopteraEndotoxinsInsecticide ResistanceLarvaZea maysAnimalsATP-Binding Cassette, Sub-Family C ProteinsBacillus thuringiensisGene Knockdown TechniquesHemolysin ProteinsInsect ProteinsPlants, Genetically ModifiedATP-Binding Cassette, Sub-Family C ProteinsATP-Binding Cassette TransportersBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisInsect ProteinsBacillus thuringiensisCorn rootwormDiabrotica virgifera virgiferaInsect resistance managementResistance mechanismsRNAi

Identifiers

PMID39733129
PMCPMC11682398

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.