Evidence map›Paper›PMID 39732674›Full record

ArticleBMC veterinary research2024

Ehrlichia sp. in dairy cattle from Bahia, Brazil: high seropositivity rates and molecular confirmation of Ehrlichia minasensis.

Antònio C Diakos, Taynar L Bezerra, Waléria Borges-Silva, Mateus A Cedro, Rogério F de Jesus, Daniel M Aguiar, Priscila J S G Mizuuti, Tayane B S Magalhães, Nathalia A Pereira, Luís F P Gondim

Abstract read
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Antònio C DiakosDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Taynar L BezerraDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Waléria Borges-SilvaDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Mateus A CedroDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Rogério F de JesusDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Daniel M AguiarLaboratório de Virologia e Rickettsioses, Hospital Veterinário da Universidade Federal de Mato Grosso (HOVET-UFMT), Av. Fernando Correa da Costa 2367, Cuiabá, Mato Grosso, CEP 78090-900, Brazil.
Priscila J S G MizuutiLaboratório de Virologia e Rickettsioses, Hospital Veterinário da Universidade Federal de Mato Grosso (HOVET-UFMT), Av. Fernando Correa da Costa 2367, Cuiabá, Mato Grosso, CEP 78090-900, Brazil.
Tayane B S MagalhãesLaboratório de Virologia e Rickettsioses, Hospital Veterinário da Universidade Federal de Mato Grosso (HOVET-UFMT), Av. Fernando Correa da Costa 2367, Cuiabá, Mato Grosso, CEP 78090-900, Brazil.
Nathalia A PereiraLaboratório de Virologia e Rickettsioses, Hospital Veterinário da Universidade Federal de Mato Grosso (HOVET-UFMT), Av. Fernando Correa da Costa 2367, Cuiabá, Mato Grosso, CEP 78090-900, Brazil.
Luís F P GondimDepartamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil. pita@ufba.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 311051/2019-7
6 · The paper itself

Abstract

backgroundEhrlichia spp. are obligate intracytoplasmic Gram-negative tickborne bacteria from the Anaplasmataceae family. Ehrlichiosis is considered an emerging disease in humans and animals. Studies on bovine ehrlichiosis are scarce in Northeastern Brazil. Therefore, the objectives of this work were to determine the frequency of antibodies anti-Ehrlichia sp. in dairy cattle herds in the Feira de Santana microregion, Bahia, and to confirm infection through PCR and nucleotide sequencing in selected seropositive animals.

resultsBlood samples were collected from 256 clinically healthy adult cattle from four dairy farms. Plasma from each animal was tested for Ehrlichia sp. using an indirect immunofluorescence antibody test (IFAT) and a 1:40 cutoff. Of the 256 tested samples, 249 (97.26%) were seropositive for Ehrlichia sp. Forty blood samples (10 from each farm) from IFAT-positive cattle were examined by a PCR for Ehrlichia sp. and resulted in 13 infected animals; DNA samples from six PCR-positive cows were sequenced and matched 100% with Ehrlichia minasensis.

conclusionsThe results represent the first serological and molecular evidence of E. minasensis in cattle from Bahia state and northeastern Brazil. Despite the limited number of tested cattle, the high frequency of seropositive animals in four farms suggests that Ehrlichia sp. is widely disseminated in the studied microregion. Considering that the infected cattle were asymptomatic, it is possible that they developed protective immunity against Ehrlichia sp.

Indexed as

Cattle DiseasesEhrlichiaEhrlichiosisPolymerase Chain ReactionAnimalsAntibodies, BacterialBrazilCattleDairyingFemaleFluorescent Antibody Technique, IndirectSeroepidemiologic StudiesAntibodies, BacterialAntibodyBovineEhrlichiosisPCRSequencingSerology

Identifiers

PMID39732674
PMCPMC11681724

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.