Evidence map›Paper›PMID 39731913›Full record

ArticleDevelopmental cell2025

Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice.

Pin-Ji Lei, Katarina J Ruscic, Kangsan Roh, Johanna J Rajotte, Meghan J O'Melia, Echoe M Bouta, Marla Marquez, Ethel R Pereira, Ashwin S Kumar, Mohammad S Razavi and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Regulation of Collecting Lymphatic Vessel Contractile Function by TRPV4 Channels.Arteriosclerosis, thrombosis, and vascular biology · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Risk factors for severity of breast cancer-related lymphedema.Radiation oncology (London, England) · 2025
    Observational
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Pin-Ji LeiEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Katarina J RuscicEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Kangsan RohEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Corrigan-Minehan Heart Center and Cardiology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Johanna J RajotteEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Roswell Park Cancer Institute, Buffalo, NY 14203, USA.
Meghan J O'MeliaEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Echoe M BoutaEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Marla MarquezEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Ethel R PereiraEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Ashwin S KumarEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Mohammad S RazaviEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Hengbo ZhouEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
Lutz MenzelEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Liqing HuangDepartment of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Heena KumraEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Mark DuquetteEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Peigen HuangEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
James W BaishDepartment of Biomedical Engineering, Bucknell University, Lewisburg, PA 17837, USA.
Lance L MunnEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Natasza A KurpiosDepartment of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Jessalyn M UbellackerDepartment of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.
Timothy P PaderaEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Electronic address: tpadera@mgh.harvard.edu.

Funding

Research Training for AnesthetistsT32GM007592 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI STUART A FORMAN · 1985 to 2026
$11.8M
Systems biology of lymphatic transportR01HL128168 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI MUNN, LANCE L., PADERA, TIMOTHY P · 2015 to 2019
$2.9M
Targeting lymph node metastases to prevent cancer progressionR01CA214913 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2017 to 2022
$2.0M
Targeting lymph node metastases to block cancer progressionR01CA284372 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI TIMOTHY P PADERA · 2023 to 2026
$2.0M
Systems Biology of Antigen and T-Cell Transport in Cancer ImmunotherapyR01CA284603 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI Lance L. Munn, TIMOTHY P PADERA · 2023 to 2026
$1.9M
Characterization of lymphatic contraction during infectionR21AI097745 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2012 to 2013
$480k
Reversing aging-induced lymphatic dysfunction to improve immune functionR21AG072205 · NIA · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2022 to 2023
$458k
A mechanistic dissection of the role of Eya3 in breast cancer metastasisK00CA234940 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI ZHOU, HENGBO · 2020 to 2023
$405k
Enhanced antigen-lymphocyte interactions to improve immune checkpoint blockade in breast cancerF32CA275298 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI O'MELIA, MEGHAN · 2022 to 2024
$213k
Regeneration and remodeling of collecting lymphatic vessels post-injuryF32HL156654 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI RAZAVI RIZI, MOHAMMAD · 2021 to 2024
$212k
NCI NIH HHS F32 CA275298NCI NIH HHS K00 CA234940NCI NIH HHS R01 CA214913NCI NIH HHS R01 CA284372NCI NIH HHS R01 CA284603NHLBI NIH HHS F32 HL156654NHLBI NIH HHS R01 HL128168NIAID NIH HHS R21 AI097745NIA NIH HHS R21 AG072205NIGMS NIH HHS T32 GM007592
6 · The paper itself

Abstract

Lymphatic muscle cells (LMCs) within the wall of collecting lymphatic vessels exhibit tonic and autonomous phasic contractions, which drive active lymph transport to maintain tissue-fluid homeostasis and support immune surveillance. Damage to LMCs disrupts lymphatic function and is related to various diseases. Despite their importance, knowledge of the gene transcriptional signatures in LMCs and how they relate to lymphatic function in normal and disease contexts is largely missing. We have generated a comprehensive transcriptional single-cell atlas-including LMCs-of peripheral collecting lymphatic vessels from mice across the lifespan. We identified genes that distinguish LMCs from other types of muscle cells, characterized the phenotypical and transcriptomic changes in LMCs in aged vessels, and identified a proinflammatory microenvironment that suppresses the contractile apparatus in LMCs from advanced-aged mice. Our findings provide a valuable resource to accelerate future research for the identification of potential drug targets on LMCs to improve lymphatic vessel function.

Indexed as

AgingLymphatic VesselsMyocytes, Smooth MuscleTranscriptomeAnimalsFemaleMaleMiceMice, Inbred C57BLagingion channellymphatic muscle cellslymphatic pumpingsingle-cell RNA sequencing

Identifiers

PMID39731913
PMCPMC11981864

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.