Evidence map›Paper›PMID 39731326›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

A Modular Engineered DNA Nanodevice for Precise Profiling of Telomerase RNA Location and Activity.

Shi-Yi Zhang, Jian Lv, Ze-Rui Zhou, Peter X Geng, Da-Wei Li, Ruo-Can Qian, Huangxian Ju

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shi-Yi ZhangKey Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.ORCID https://orcid.org/0009-0008-8983-0997
Jian LvKey Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Ze-Rui ZhouKey Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Peter X GengDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, 100871, P. R. China.
Da-Wei LiKey Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Ruo-Can QianKey Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.ORCID https://orcid.org/0000-0002-1039-9866
Huangxian JuState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China.ORCID https://orcid.org/0000-0002-6741-5302

Funding

Fundamental Research Funds for Central Universities of the Central South UniversityNational Natural Science Foundation of China 21977031Science and Technology Commission of Shanghai Municipality 2018SHZDZX03Science and Technology Commission of Shanghai Municipality 22ZR1416800Science and Technology Commission of Shanghai Municipality 24DX1400200Shanghai Science and Technology Committee 2018SHZDZX03Shanghai Science and Technology Committee 22ZR1416800Shanghai Science and Technology Committee 23ZR1416100
6 · The paper itself

Abstract

Increased telomerase activity has been considered as a conspicuous sign of human cancers. The catalytic cores of telomerase involve a reverse transcriptase and the human telomerase RNA (hTR). However, current detection of telomerase is largely limited to its activity at the tissue and single-cell levels. To reveal the precise distribution of subcellular hTR and telomerase activity, here a modular engineered DNA nanodevice (DNA-ND) is designed capable of imaging hTR and telomerase activity in cytoplasm and nucleus, enabling colocalization analysis. DNA-ND is a modular DNA complex comprising hTR and telomerase activity detection modules, which respectively sense intercellular hTR and telomerase activity via target-sensitive allosteric transition of DNA switches, actuating orthogonal activation of fluorescence signals to achieve in situ co-imaging of hTR and telomerase activity. By integrating DNA-ND with specific localized signals, the DNA-ND based precise profiling of colocalization of hTR and telomerase activity in different cell lines as well as their dynamic changes during pharmacological interventions is demonstrated. Notably, the results suggest that the locations of hTR and telomerase activity are not exactly overlapped, indicating the influence of intracellular environment on the binding of hTR to telomerase.

Indexed as

DNARNATelomeraseHumansDNARNATelomerasetelomerase RNADNA engineeringhuman telomerase RNAtelomerase activity

Identifiers

PMID39731326
PMCPMC11831533

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.