Evidence map›Paper›PMID 39730944›Full record

ArticleJournal of assisted reproduction and genetics2025

FXYD1 was identified as a hub gene in recurrent miscarriage and involved in decidualization via regulating Na/K-ATPase activity.

Jie Gan, Long Yang, Shu-Han Yang, Wen-Wen Gu, Yan Gu, Yan Shi, Jia-Xin Shi, Hao-Ran Xu, Ya-Wei Xin, Xuan Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jie Gan *Shanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Long Yang *Shanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Shu-Han YangShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Wen-Wen GuShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Yan GuThe Second Hospital of Tianjin Medical University, Tianjin, 300221, China.
Yan ShiShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Jia-Xin ShiShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Hao-Ran XuShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China.
Ya-Wei XinThe Second Hospital of Tianjin Medical University, Tianjin, 300221, China.
Xuan ZhangShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China. xuanzhang@sibpt.com.ORCID http://orcid.org/0000-0003-0544-4519
Jian WangShanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of ReproductionRegulation,Shanghai Institute for Biomedical and Pharmaceutical Technologies,Medical School, Fudan University, Shanghai, 200237, China. wangjian@sibpt.com.

Funding

National Natural Science Foundation of China 82371686Natural Science Foundation of Shanghai Municipality No.23ZR1457000
6 · The paper itself

Abstract

purposeRecurrent miscarriage (RM) is a distressing and complicated adverse pregnancy outcome. It is commonly recognized that insufficient decidualization could result in RM, but the molecular mechanisms of decidual impairment are still not fully understood. Thus, this study aimed to identify novel key genes potentially involved in RM and explore their roles played in endometrial decidualization.

methodsInitially, a combinative analysis of decidual and mid-secretory endometrial transcriptomes was performed to discover hub genes involved in the etiology of RM. And the expression levels of hub genes were evaluated in both primary decidual stromal cells (DSCs) and decidual tissues. Subsequently, the immortalized human endometrial cell line, T-HESCs, was used to investigate whether FXYD1 overexpression affects decidualization by regulating Na/K-ATPase activity.

resultsFXYD domain containing ion transport regulator 1 (FXYD1) was identified as a hub gene in the pathogenesis of RM through various bioinformatic methods. Abnormally increased FXYD1 expression was observed in DSCs and decidual tissues from RM patients compared to that of the normal group. Furthermore, in vitro decidualization was obviously inhibited by the overexpression of FXYD1. Additionally, Na/K-ATPase activity was significantly elevated during decidualization, whereas overexpression of FXYD1 reduced Na/K-ATPase activity. Bufalin, a Na/K-ATPase inhibitor, showed an effectively inhibitory effect on decidualization.

conclusionsCollectively, FXYD1 was discovered as a hub gene associated with RM, and its expression levels in RM patients were significantly upregulated. Increased FXYD1 expression might lead to decidualization defects by reducing Na/K-ATPase activity, of which presented a novel prospective treatment target for RM.

Indexed as

Abortion, HabitualDeciduaMembrane ProteinsPhosphoproteinsSodium-Potassium-Exchanging ATPaseAdultEndometriumFemaleHumansPregnancyStromal CellsMembrane ProteinsphospholemmanPhosphoproteinsSodium-Potassium-Exchanging ATPaseDecidualizationFXYDHub genesNa/K-ATPaseRecurrent miscarriage

Identifiers

PMID39730944
PMCPMC11871252

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.