ArticleScientific reports2024
In Vitro conservation and genetic diversity analysis of rare species Ribes janczewskii.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Micropropagation and ex vitro acclimatization of Lonicera caerulea var. altaica: molecular identification and protocol optimization.Scientific reports · 2026Article
- In Vitro Micropropagation of Oca (Plants (Basel, Switzerland) · 2025Article
- Genome Editing by Grafting.International journal of molecular sciences · 2025Review
- Geobotanical Study and Preservation of Rare and Endangered Rosaceae Species.Plants (Basel, Switzerland) · 2025Article
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9 authors.
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Abstract
Ribes janczewskii is a rare and valuable plant known for its resistance to spring frosts, pests, and diseases. It is used in hybridization to develop resistant currant varieties but is on the verge of extinction, listed in Kazakhstan Red Book. This study developed a micropropagation and slow-growth storage protocol for conservation. Genotypes were identified through DNA barcode analysis (rbcL, ITS, and matK) and sequences uploaded to the National Center for Biotechnology Information database. Genetic diversity was assessed using iPBS primers, generating 98 fragments with 88-94% polymorphic bands. Biochemical analysis of fruits showed vitamin C content from 4.64 to 5.61 mg/100 g, vitamin E from 2.26 to 3.16 mg/100 g, vitamin B5 from 3.18 to 4.93 mg/100 g, and quercetin up to 12.5 mg/100 g. Micropropagation stages were optimized with 12% hydrogen peroxide for surface sterilization, achieving up to 73.3% explant viability. Effective hormonal combinations for in vitro culture included WPM with BAP 0.2 mg L
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