Evidence map›Paper›PMID 39730700›Full record

ArticleScientific reports2024

Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens.

Camilla V Pires, Jyotsna Chawla, Lauriane Sollelis, Jenna Oberstaller, Min Zhang, Chengqi Wang, Justin Gibbons, Julian C Rayner, Thomas D Otto, Matthias Marti and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Camilla V Pires *Center for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Jyotsna Chawla *Center for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Lauriane SollelisInstitute of Parasitology Zurich, VetSuisse Faculty, University of Zurich, Zurich, Switzerland.
Jenna OberstallerCenter for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Min ZhangCenter for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Chengqi WangCenter for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Justin GibbonsCenter for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA.
Julian C RaynerCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.
Thomas D OttoInstitute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Matthias MartiInstitute of Parasitology Zurich, VetSuisse Faculty, University of Zurich, Zurich, Switzerland.
John H AdamsCenter for Global Health and Inter-Disciplinary Research, College of Public Health, University of South Florida, Tampa, FL, USA. ja2@usf.edu.

Funding

Chemogenomic Profiling of Plasmodium Falciparum Responses and ResistanceR01AI117017 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI ADAMS, JOHN H · 2015 to 2025
$6.9M
Discovering the essential genome of Plasmodium falciparumR01AI130171 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI ADAMS, JOHN H · 2018 to 2021
$2.8M
Phenotype-based Screens to Identify Genetic Factors Associated with Gametocyte Development in Plasmodium falciparumR01AI187153 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI John H Adams · 2025 to 2026
$1.4M
National Institute of Allergy and Infectious Diseases R01AI117017NIAID NIH HHS R01 AI117017NIAID NIH HHS R01 AI130171NIAID NIH HHS R01 AI187153Wellcome Trust
6 · The paper itself

Abstract

Successful transmission of Plasmodium falciparum from one person to another relies on the complete intraerythrocytic development of non-pathogenic sexual gametocytes infectious for anopheline mosquitoes. Understanding the genetic factors that regulate gametocyte development is vital for identifying transmission-blocking targets in the malaria parasite life cycle. Toward this end, we conducted a forward genetic study to characterize the development of gametocytes from sexual commitment to mature stage V. We described a new analysis pipeline for the piggyBac transposon-based mutagenesis phenotypic screen to identify genes that influence both early and late gametocyte stages. We classified individual mutants that increased or decreased parasite abundance as the hypoproducer and hyperproducer phenotypes, respectively, revealing distinctive temporal genetic factors early and late in the sexual development cycle. The study identifies that disruption in factors involved in transcription, protein trafficking and DNA repair are associated with decreasing gametocyte production, while modifications in phosphatase activity are linked to hyperproduction of gametocytes. Our study provides an optimized approach on genotype-phenotype evaluation, offering a new resource for understanding potential targets for therapeutic intervention strategies to disrupt transmission.

Indexed as

GametogenesisPhenotypePlasmodium falciparumAnimalsHumansLife Cycle StagesMalaria, FalciparumProtozoan ProteinsProtozoan Proteins

Identifiers

PMID39730700
PMCPMC11680961

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.