Evidence map›Paper›PMID 39729990›Full record

ArticleStem cell reports2025

Heparan sulfate regulates the fate decisions of human pluripotent stem cells.

Deepsing Syangtan, Deena Al Mahbuba, Sayaka Masuko, Qiao Li, Andrew C Elton, Yefim Zaltsman, Paul J Wrighton, Ke Xia, Xiaorui Han, Yilan Ouyang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Deepsing SyangtanDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Deena Al MahbubaDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Sayaka MasukoDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Qiao LiDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Andrew C EltonSchool of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.
Yefim ZaltsmanDepartment of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.
Paul J WrightonDepartment of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.
Ke XiaDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th St., Troy, NY 12180, USA.
Xiaorui HanDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th St., Troy, NY 12180, USA.
Yilan OuyangDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th St., Troy, NY 12180, USA.
Fuming ZhangDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th St., Troy, NY 12180, USA.
Robert J LinhardtDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th St., Troy, NY 12180, USA.
Laura L KiesslingDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA; Broad Institute of MIT and Harvard, 415 Main St, Cambridge, MA 02142, USA; Koch Institute for Integrative Cancer Research at MIT, 500 Main St, Cambridge, MA 02142, USA. Electronic address: kiesslin@mit.edu.

Funding

Synthetic Ligands for Modulating Immune Cell ResponsesR01AI055258 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KIESSLING, LAURA L · 2003 to 2024
$7.8M
PROBES OF CARBOHYDRATE FUNCTIONR01GM049975 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI KIESSLING, LAURA L · 1999 to 2016
$5.2M
Advanced sequencing as a novel diagnostic tool to discover strain-level variation and function of mucosal-adherent bacteria contributing to IBDK01DK119582 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI WANG, JEREMY R · 2019 to 2023
$754k
Glycomics-driven diagnostics for Candida aurisR21AI156573 · NIAID · RENSSELAER POLYTECHNIC INSTITUTE · PI CHATURVEDI, VISHNU, ZHANG, FUMING · 2021 to 2022
$454k
Glycosaminoglycans as key regulators of stem cell self-renewalF32GM111014 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI MASUKO, SAYAKA · 2014 to 2015
$109k
NIAID NIH HHS R01 AI055258NIAID NIH HHS R21 AI156573NIDDK NIH HHS K01 DK119582NIGMS NIH HHS F32 GM111014NIGMS NIH HHS R01 GM049975
6 · The paper itself

Abstract

Heparan sulfate (HS) is an anionic polysaccharide generated by all animal cells, but our understanding of its roles in human pluripotent stem cell (hPSC) self-renewal and differentiation is limited. We derived HS-deficient hPSCs by disrupting the EXT1 glycosyltransferase. These EXT1

Indexed as

Cell DifferentiationCell LineageHeparan SulfatePluripotent Stem CellsActivinsExostosin 1Fibroblast Growth Factor 2HumansMesodermMotor NeuronsN-AcetylglucosaminyltransferasesSignal TransductionActivinsExostosin 1Fibroblast Growth Factor 2Heparan SulfateN-Acetylglucosaminyltransferasesbasic fibroblast growth factorbFGFCRISPRectodermEXT1glycanglycosyltransferaseheparan sulfatemesendodermneuronnodal

Identifiers

PMID39729990
PMCPMC11784485

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.