Evidence map›Paper›PMID 39729489›Full record

ArticlePloS one2024

Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis.

Marwa A Ali, Olfat G Shaker, Eman M Ezzat, El Shaimaa Gomaa Ali, Mahmoud I Aboelor, Mona I Ahmed, Essam A Hassan, Doaa Y Ali, Heba Mostafa Ahmed, Abeer A Khalefa and 3 more

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Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. SARS-CoV-2 reshapes mNAR molecular medicine · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Marwa A AliFaculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.ORCID 0000-0002-4007-7868
Olfat G ShakerFaculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Cairo University, Cairo, Egypt.
Eman M EzzatFaculty of Medicine, Department of Internal Medicine, Fayoum University, Fayoum, Egypt.
El Shaimaa Gomaa AliFaculty of Medicine, Department of Medical Microbiology and Immunology, Fayoum University, Fayoum, Egypt.
Mahmoud I AboelorFaculty of Medicine, Department of Radio-Diagnosis, Fayoum University, Fayoum, Egypt.
Mona I AhmedFaculty of Medicine, Department of Chest Disease, Fayoum University, Fayoum, Egypt.
Essam A HassanFaculty of Medicine, Department of Tropical Medicine, Fayoum University, Fayoum, Egypt.
Doaa Y AliFaculty of Medicine, Department of Clinical and Chemical Pathology, Fayoum University, Fayoum, Egypt.
Heba Mostafa AhmedFaculty of Medicine, Department of Clinical and Chemical Pathology, Fayoum University, Fayoum, Egypt.
Abeer A KhalefaFaculty of Medicine, Department of Physiology, Zagazig University, Zagazig, Egypt.
Rasha A HusseinFaculty of Medicine, Department of Medical Microbiology and Immunology, Zagazig University, Zagazig, Egypt.
Ahmed Fathy ElkhateebFaculty of Medicine, Department of Critical Care, Fayoum University, Fayoum, Egypt.
Esam Ali MohamedFaculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies for the disease.

aimOur study aimed to measure NEAT-1, miR-374b-5p, and IL6 in the serum of COVID-19 patients, demonstrating the correlation between target genes to explore the possible relationship between them. Also, the association between target genes and patients' clinical findings and radiological severity indices will be explored. PATIENTS AND

methodsThe current study included 48 COVID-19-infected individuals and 40 controls. Quantitative real-time PCR (qPCR) was performed to detect lncRNA NEAT-1 and miRNA374b-5p fold change (FC) in the participants' sera. Enzyme-Linked Immune Sorbent Assay (ELISA) is used to detect IL6.

resultsOur results showed statistical significance with lower levels of (NEAT-1) [ median (range) = 0.08 (0.001-0.602)], and (miR374b-5p) [ median (range) = 0.14 (.01-7.16)] while higher IL-6 levels [ median (range) = 41.3 (7.2-654) pg/ml] when compared to controls with p-value <0.001. Serum level of NEAT-1 correlates negatively with IL-6 level (r = -.317, P = .008). ROC curve analysis revealed that sensitivity and specificity tests for NEAT-1 and IL-6 levels in the diagnosis of cases illustrated a sensitivity of (100% and 97.9%) and a specificity of (85% and 100%) at cut-off values (0.985 and 12.55), respectively. In comparison, miR374b-5p showed sensitivity and specificity of around 85% in distinguishing COVID-19 patients from controls. No significant association was detected between target genes and radiological severity indices.

conclusionsOur study is the first to detect decreased NEAT-1 and miR374b-5p expression in COVID-19 patients' serum. There was also an increase in IL6 levels. There is a negative correlation between NEAT-1 and IL6 in COVID-19 patients.

Indexed as

COVID-19Interleukin-6MicroRNAsRNA, Long NoncodingSARS-CoV-2AdultAgedBiomarkersCase-Control StudiesFemaleHumansMaleMiddle AgedPrognosisROC CurveBiomarkersIL6 protein, humanInterleukin-6MicroRNAsNEAT1 long non-coding RNA, humanRNA, Long Noncoding

Identifiers

PMID39729489
PMCPMC11676515

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.