Evidence map›Paper›PMID 39729280›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Heterologous Expression of Secreted Proteins from Phytophthora in Pichia pastoris Followed by Protein Purification by Immobilized Metal Ion Affinity (IMAC).

Mariana Tarallo, Max Hayhurst, Trevor S Loo, Monica L Gerth, Rosie E Bradshaw

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mariana TaralloBioprotection Aotearoa, School of Food Technology and Natural Sciences, Massey University, Palmerston North, New Zealand.
Max HayhurstSchool of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Trevor S LooBioprotection Aotearoa, School of Food Technology and Natural Sciences, Massey University, Palmerston North, New Zealand.
Monica L GerthSchool of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Rosie E BradshawBioprotection Aotearoa, School of Food Technology and Natural Sciences, Massey University, Palmerston North, New Zealand. R.E.Bradshaw@massey.ac.nz.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes the protocol for heterologous expression of Phytophthora proteins in the yeast Pichia pastoris. Two methods to prepare the constructs for expression are described, using two different strains of P. pastoris, as well as methods for protein expression and purification by immobilized metal ion affinity (IMAC). The combined protocol was used to successfully produce a Phytophthora pluvialis small, secreted cell death elicitor protein and a Phytophthora agathidicida glycoside hydrolase 12, PaXEG1, also a cell death-inducing protein.

Indexed as

PhytophthoraChromatography, AffinityGene ExpressionMetalsRecombinant ProteinsSaccharomycetalesMetalsRecombinant ProteinsHeterologous protein productionImmobilized metal ion affinityProtein purificationYeast transformation

Identifiers

PMID39729280

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.