Evidence map›Paper›PMID 39729270›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Fluorescence In Situ Hybridization Protocol for Visualization of Oomycetes In Vitro and In Planta.

Glen Groben, Catalina Salgado-Salazar, Jo Anne Crouch

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Glen GrobenUnited States Department of Agriculture, Agricultural Research Service, Foreign Disease/Weed Science Research Unit, Frederick, MD, USA.
Catalina Salgado-SalazarUnited States Department of Agriculture, Agriculture Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, Beltsville, MD, USA.
Jo Anne CrouchUnited States Department of Agriculture, Agricultural Research Service, Foreign Disease/Weed Science Research Unit, Frederick, MD, USA. joanne.crouch@usda.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence in situ hybridization enables the visualization of organisms in the environment without having to culture them. Here, we describe a FISH protocol to visualize oomycete structures (mycelia, sporangiophores, sporangia, and oospores) directly as well as from colonized plant material. The protocol utilizes organic compounds with low toxicities and does not require a permeabilization step. The protocol was used to visualize impatiens downy mildew Plasmopara destructor sporangiophores colonizing an Impatiens walleriana leaf.

Indexed as

In Situ Hybridization, FluorescenceOomycetesPlant DiseasesPlant LeavesCulture-independent visualizationFISHFluorescence in situ hybridization

Identifiers

PMID39729270

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.