Optimization of Cholesterol-Loaded Cyclodextrin Combined with Soybean Lecithin as a Cryoprotectant for Rooster Sperm.

Mengqian He, Jiehuan Xu, Lingwei Sun, Caifeng Wu, Shushan Zhang, Jun Gao, Defu Zhang, Jianjun Dai

Abstract read

In one paragraph

Article in Veterinary sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mengqian HeShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Jiehuan XuShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Lingwei SunShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Caifeng WuShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Shushan ZhangShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.ORCID 0000-0002-6762-1931

Jun GaoShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.ORCID 0000-0002-2372-4445

Defu ZhangShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Jianjun DaiShanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There are many applications of soybean lecithin (SL) and cholesterol-loaded cyclodextrin (CLC) in sperm freezing processes. To the best of our knowledge, there have been few cases of the combined use of SL and CLC in freezing rooster semen. We investigated the effects of CLC, SL, and their combination on rooster sperm cryodamage. Three experiments were conducted: experiment 1, SL (0.5%, 1%, 1.5%, 2.0%); experiment 2, CLC (1.25 mg, 2.5 mg, 5 mg, 10 mg, 15 mg); and experiment 3, CLC + SL (2.5 mg + 0.25%, 2.5 mg + 0.5%, 2.5 mg + 1%, 2.5 mg + 1.5%). Semen samples were cryopreserved using a programmed cryostat, followed by the determination of post-thaw sperm quality, antioxidant indices, and hatching. The results showed that the combination of 2.5 mg CLC + 0.5% SL had the most significant synergistic effect on cryodamage, and the viability (56.69%), motility (54.35%), mitochondrial activity (54.23%), plasma membrane integrity (53.52%), acrosome integrity (54.71%), and antioxidant activity (MDA concentration: 5.65 nmol/mL; SOD activity: 152.73 U/mL) were significantly greater than those of the other combinations (

Indexed as

artificial inseminationCLCroostersemen cryopreservationSL

Identifiers

PMID39728987

DOI10.3390/vetsci11120647

PMCPMC11680394

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.