ArticleThe Journal of cell biology1985
Isolation of an actin-binding protein from membranes of Dictyostelium discoideum.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect.Eukaryotic cell · 2002Article
- Linking microfilaments to intracellular membranes: the actin-binding and vesicle-associated protein comitin exhibits a mannose-specific lectin activity.The EMBO journal · 1996Article
- The Dictyostelium cytoskeleton.Experientia · 1995Review
- The actin-binding protein comitin (p24) is a component of the Golgi apparatus.The Journal of cell biology · 1993Article
- The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly.The Journal of cell biology · 1993Article
- Actin binding proteins--lipid interactions.Journal of muscle research and cell motility · 1991Review
- Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein.The Journal of cell biology · 1990Article
- F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes.The Journal of cell biology · 1987Article
- Using antibodies against Dictyostelium membranes to identify an actin-binding membrane protein.The Journal of cell biology · 1987Article
- Interaction of the cytoskeleton with the plasma membrane.The Journal of membrane biology · 1987Review
- The membrane topography of ecto-5'-nucleotidase in rat hepatocytes.The Biochemical journal · 1986Article
- Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum.The Journal of cell biology · 1986Article
- Cytochalasin B affects the structural polarity of statocytes from cress roots (Lepidium sativum L.).Protoplasma · 1985Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We prepared a probe of radiolabeled, glutaraldehyde cross-linked filamentous actin (F-actin) to study binding of actin to membranes of Dictyostelium discoideum. The probe bound to membranes or detergent extracts of membranes with a high affinity and in a saturable manner. The binding could be reduced by boiling of either the actin probe or the membranes, or by addition of excess native F-actin, but not by addition of an equivalent amount of bovine serum albumin, to the assay. The probe labeled several proteins when used to overlay sodium dodecyl sulfate gels of Dictyostelium membranes. One of these labeled proteins was a 24,000-mol-wt protein (p24), which was soluble only in the presence of a high concentration of sodium deoxycholate (5%, wt/vol) at room temperature or above. The p24 was purified by selective detergent extraction and column chromatography. When tested in a novel two-phase binding assay, p24 bound both native monomeric actin (G-actin) and F-actin in a specific manner. In this assay, G-actin bound p24 with a submicromolar affinity.
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Registered trials
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