Evidence map›Paper›PMID 39727993›Full record

ArticleCurrent issues in molecular biology2024

Construction and Validation of CRISPR/Cas Vectors for Editing the

Marcelly Santana Mascarenhas, Fernanda Dos Santos Nascimento, Luana Maria Pacheco Schittino, Livia Batista Galinari, Lucymeire Souza Morais Lino, Andresa Priscila de Souza Ramos, Leandro Eugenio Cardamone Diniz, Tiago Antônio de Oliveira Mendes, Claudia Fortes Ferreira, Janay Almeida Dos Santos-Serejo and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Progress in Molecular Plant Science (2023-2025).Current issues in molecular biology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marcelly Santana MascarenhasDepartment of Biological Sciences, Feira de Santana State University, Feira de Santana 44036-900, BA, Brazil.ORCID 0000-0002-8468-5099
Fernanda Dos Santos NascimentoEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0003-3033-1604
Luana Maria Pacheco SchittinoDepartment of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa 36507-900, MG, Brazil.ORCID 0009-0001-5261-7016
Livia Batista GalinariDepartment of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa 36507-900, MG, Brazil.ORCID 0009-0003-2535-2483
Lucymeire Souza Morais LinoEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0001-6400-8034
Andresa Priscila de Souza RamosEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0002-3677-4855
Leandro Eugenio Cardamone DinizEmbrapa Soja, Rodovia Carlos João Strass, Londrina 86085-981, PR, Brazil.ORCID 0000-0003-1980-9945
Tiago Antônio de Oliveira MendesDepartment of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa 36507-900, MG, Brazil.
Claudia Fortes FerreiraEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0001-5476-2236
Janay Almeida Dos Santos-SerejoEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0003-3793-1792
Edson Perito AmorimEmbrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.ORCID 0000-0001-9086-7385

Funding

Bill and Melinda Gates Foundation - Accelerated Breeding of Better Bananas OPP1093845IITA - Accelerated Breeding of Better Bananas OPP1093845
6 · The paper itself

Abstract

Bananas and plantains are important staple food crops affected by biotic and abiotic stresses. The gene editing technique via Clustered Regularly Interspaced Short Palindromic Repeats associated with the Cas protein (CRISPR/Cas) has been used as an important tool for development of cultivars with high tolerance to stresses. This study sought to develop a protocol for the construction of vectors for gene knockout. Here we use the phytoene desaturase (

Indexed as

bananasCRISPR technologyknockoutphytoene desaturasePrata-Anãpromotervector

Identifiers

PMID39727993
PMCPMC11674420

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.