Development of RT h-CLAT, a Rapid Assessment Method for Skin Sensitizers Using THP-1 Cells as a Biosensor.

Hiroki Koyama, Ayami Maeda, Peiqi Zhai, Keiichiro Koiwai, Kouichi Kurose

Abstract read

In one paragraph

Article in Biosensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Hiroki KoyamaDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Ayami MaedaDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Peiqi ZhaiDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Keiichiro KoiwaiDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.ORCID 0000-0002-8890-8229

Kouichi KuroseDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.ORCID 0000-0002-7042-0153

Funding

JSPS KAKENHI JP 18K02220 and 21K02070

6 · The paper itself

Abstract

In recent years, in vitro skin sensitization assays have been recommended as animal-free alternatives for the safety assessment of cosmetics and topical drugs, and these methods have been adopted in OECD test guidelines. However, existing assays remain complex and costly. To address this, we recently developed a more efficient, cost-effective, and accurate method for evaluating skin sensitizers by using immune cell-derived THP-1 cells as a biosensor, coupled with an RT-PCR-based assay. In this study, we further refined this method to enable even faster assessment of skin sensitization. By performing comprehensive RNA sequencing (RNA-Seq) analysis, we examined gene expression profiles induced by sensitizers in THP-1 cells to identify potential sensitization markers, ultimately selecting the optimal markers and conditions for evaluation. Our findings indicate that after exposing a test chemical to THP-1 cells for 5 h, measuring the expression levels of the

Indexed as

Biosensing TechniquesAllergensHumansSkinTHP-1 CellsAllergensalternative methodsbiomarkerHMOX1in vitro skin sensitization testJUNRNA-Seq analysis

Identifiers

PMID39727897

DOI10.3390/bios14120632

PMCPMC11674273

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.