Evidence map›Paper›PMID 39727786›Full record

ArticleBiomimetics (Basel, Switzerland)2024

The Influence of Physiological Blood Clot on Osteoblastic Cell Response to a Chitosan-Based 3D Scaffold-A Pilot Investigation.

Natacha Malu Miranda da Costa, Hilary Ignes Palma Caetano, Larissa Miranda Aguiar, Ludovica Parisi, Benedetta Ghezzi, Lisa Elviri, Leonardo Raphael Zuardi, Paulo Tambasco de Oliveira, Daniela Bazan Palioto

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In one paragraph

Article in Biomimetics (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Natacha Malu Miranda da CostaDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Hilary Ignes Palma CaetanoDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Larissa Miranda AguiarDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Ludovica ParisiLaboratory for Oral Molecular Biology, Department of Orthodontics and Dentofacial Orthopedics, University of Bern, Freiburgstrasse 3, 3010 Bern, Switzerland.
Benedetta GhezziCentro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, University of Parma, Via Gramsci 14, 43126 Parma, Italy.ORCID 0000-0002-8922-2915
Lisa ElviriIstituto dei Materiali per l'Elettronica ed il Magnetismo, Consiglio Nazionale Delle Ricerche, Parco Area Delle Scienze 37/A, 43124 Parma, Italy.ORCID 0000-0002-5988-618X
Leonardo Raphael ZuardiDepartment of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.
Paulo Tambasco de OliveiraDepartment of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.ORCID 0000-0002-6662-0486
Daniela Bazan PaliotoDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil.ORCID 0000-0003-1913-0895

Funding

Coordination of Superior Level Staff Improvement 33002029034P-7The São Paulo Research Foundation 18/12036-3The São Paulo Research Foundation 18/16925-7
6 · The paper itself

Abstract

backgroundThe use of ex vivo assays associated with biomaterials may allow the short-term visualization of a specific cell type response inserted in a local microenvironment. Blood is the first component to come into contact with biomaterials, providing blood clot formation, being substantial in new tissue formation. Thus, this research investigated the physiological blood clot (PhC) patterns formed in 3D scaffolds (SCAs), based on chitosan and 20% beta-tricalcium phosphate and its effect on osteogenesis. Initially, SCA were inserted for 16 h in rats calvaria defects, and, after that, osteoblasts cells (OSB; UMR-106 lineage) were seeded on the substrate formed. The groups tested were SCA + OSB and SCA + PhC + OSB. Cell viability was checked by MTT and mineralized matrix formation in OSB using alizarin red (ARS). The alkaline phosphatase (ALP) and bone sialoprotein (BSP) expression in OSB was investigated by indirect immunofluorescence (IF). The OSB and PhC morphology was verified by scanning electron microscopy (SEM).

resultsThe SCA + PhC + OSB group showed greater cell viability (

conclusionsThese findings show the feasibility of using PhC in ex vivo assays. Additionally, its inclusion in the experiments resulted in a change in OSB behavior when compared to in vitro assays. This "closer to nature" environment can completely change the scenario of a study.

Indexed as

blood clotex vivo assayhydrogelosteogenesisscaffoldthree dimensional

Identifiers

PMID39727786
PMCPMC11727562

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.