Evidence map›Paper›PMID 39727483›Full record

ArticleAntibodies (Basel, Switzerland)2024

An Improved

Hannah Chege, Samuel Githigia, James Gathumbi, Naomi Chege, Rose Ojuok, Josiah Odaba, Stephen Mwalimu, Harriet Oboge, Lucilla Steinaa, Vishvanath Nene and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antibodies (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hannah ChegeAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.ORCID 0009-0005-1984-7855
Samuel GithigiaDepartment of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Nairobi P.O. Box 29053-00625, Kenya.
James GathumbiDepartment of Veterinary Pathology, Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, Nairobi P.O. Box 29053-00625, Kenya.ORCID 0000-0002-2815-7216
Naomi ChegeAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.
Rose OjuokAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.ORCID 0000-0001-8147-9743
Josiah OdabaAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.
Stephen MwalimuAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.
Harriet ObogeAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.
Lucilla SteinaaAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.ORCID 0000-0003-3691-3971
Vishvanath NeneAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.ORCID 0000-0001-7066-4169
Anna LacastaAnimal and Human Health Program, International Livestock Research Institute, Nairobi P.O. Box 30709, Kenya.ORCID 0000-0001-8381-9844

Funding

United States Agency for International Development 7200AA20CA00022
6 · The paper itself

Abstract

backgroundImmune correlates of protection are ideal tools to predict treatment or vaccine efficacy. However, the accuracy of the immune correlate and the capability to robustly predict the outcome of a vaccine candidate are determined by the performance of the in vitro immunoassay used. Several

methodsHerein, we describe key improvements, among them, (1) the use of frozen parasites and target cells to avoid batch-to-batch variations and (2) the development of a new assay read-out based on the detection of infected cells through flow cytometry, instead of the use of Giemsa staining and microscopic evaluation, in order to improve the reproducibility of the results.

resultsThe improved seroneutralization assay is not only able to detect the individual neutralizing capacity of antibodies; it also detects the additive effect of antibody combinations.

conclusionsThis effect is described for the first time in

Indexed as

antibodiesantigenEast Coast feverseroneutralization assaysporozoitesTheileria parva

Identifiers

PMID39727483
PMCPMC11672397

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.