Evidence map›Paper›PMID 39726749›Full record

ArticlePeerJ2024

Impact of storage and extraction methods on peat soil microbiomes.

Dylan Cronin, Yueh-Fen Li, Paul Evans, Gene W Tyson, Ben J Woodcroft, Virginia I Rich, IsoGenie 2016 and 2019 Field Teams

Abstract read
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dylan Cronin *Center of Microbiome Science, Ohio State University, Columbus, Ohio, United States.
Yueh-Fen Li *Department of Microbiology, Ohio State University, Columbus, Ohio, United States.
Paul EvansSchool of Chemistry and Molecular Biosciences, The Australian Centre for Ecogenomics, University of Queensland, St Lucia, Queensland, Australia.ORCID 0000-0001-5995-2602
Gene W TysonCentre for Microbiome Research, School of Biomedical Sciences, Translational Research Institute, Queensland University of Technology, Woolloongabba, Queensland, Australia.
Ben J WoodcroftCentre for Microbiome Research, School of Biomedical Sciences, Translational Research Institute, Queensland University of Technology, Woolloongabba, Queensland, Australia.ORCID 0000-0003-0670-7480
Virginia I RichCenter of Microbiome Science, Ohio State University, Columbus, Ohio, United States.ORCID 0000-0003-0558-102X
IsoGenie 2016 and 2019 Field Teams

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Jennifer A. Woyach · 1985 to 2026
$132.3M
NCI NIH HHS P30 CA016058
6 · The paper itself

Abstract

Recovered microbial community structure is known to be influenced by sample storage conditions and nucleic acid extraction methods, and the impact varies by sample type. Peat soils store a large portion of soil carbon and their microbiomes mediate climate feedbacks. Here, we tested three storage conditions and five extraction protocols on peat soils from three physicochemically distinct habitats in Stordalen Mire, Sweden, revealing significant methodological impacts on microbial (here, meaning bacteria and archaea) community structure. Initial preservation method impacted alpha but not beta diversity, with in-field storage in LifeGuard buffer yielding roughly two-thirds the richness of in-field flash-freezing or transport from the field on ice (all samples were stored at -80 °C after return from the field). Nucleic acid extraction method impacted both alpha and beta diversity; one method (

Indexed as

BacteriaMicrobiotaSoilSoil MicrobiologyArchaeaDNA, BacterialSpecimen HandlingSwedenDNA, BacterialSoilExtractionMethodsMicrobiomePeatlandSoilStorageStordalen

Identifiers

PMID39726749
PMCPMC11670759

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.